[Q]CsCl purification of cosmid

zinc zinc at biochem-49.med.utah.edu
Thu May 18 01:50:15 EST 1995


In article <yanakamu-1805951518540001 at timac230.lab.nig.ac.jp>,
NAKAMURA Yasukazu <yanakamu at lab.nig.ac.jp> wrote:
>Hi. BioNetters,
>
>  Does anyone know any protocol for purify DNA of cosmid clones by
>equilibrium centrifugation in CsCl-EtBr gradient?  Does the protocol for
>plasmid work same?

i have done both plasmid and cosmid CsCl preps (did them for years as
a lab aide when i was an undergrad...).  i used exactly the same
protocols for both.

the only difference was the extremely poor yield of DNA from the
cosmid preps as compared to the plasmids.

-pjf

-- 
patrick finerty         --      finerty at msscc.med.utah.edu
                                pfinerty at nyx10.cs.du.edu
U of Utah biochem grad student/slave in the Bass lab
easily found at (801) 585-3110 almost anytime.   rm 207 wintrobe.



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