long distance PCR and Tth?

Tay Hou Ngee Agnes mcbtayhn at leonis.nus.sg
Tue May 16 22:04:46 EST 1995


Hi
Does anyone have any experience with the different Tth DNA polymerases 
for long-distance PCR using genomci DNA as template? I 
was trying out the Human promoterfinder kit from clontech but they had no 
enzyme in stock so I substituted Tth from promega and have blanks.
Would appreciate any advice on longdistance PCR esp with genomic dna as 
template.
Thanks a lot
Agnes



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