HELP needed in western!

WZhao80850 wzhao80850 at
Fri May 19 15:27:39 EST 1995

Hi there,
I have some problem in western blotting. I am using a high dilution of
primary antibody for western, i.e. 1:25 (10ug/ml) dilution in TBST with 1%
milk. Therefore, I have high background in my result. Is there anyway I
can reduce the background? Also, the protein bands are bended and curved
on the film. Why is that happened?
Any suggestion will be great helpful for me, thanks in advance!


Wei Zhao
WZhao80850 at or weiz at
Tel: (713)669-1091(H), (713)790-4696
Baylor College of Medicine
Houston, TX 77030

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