ampification of DNA non-methylated at Xba I sites

mpk6 MPK6 at
Fri May 19 13:01:03 EST 1995

I presume that your XbaI site overlaps with a GATC dam methylation site.
XbaI cleavage is blocked by 'overlapping' dam methylation, not dam
methylation in general. Also, I have successfully introduced a number of methyl
ated DNAs into the NEB strain, GM2163 without any trouble.

Good luck,

Mike Kladde
PennState University

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