Hi...I dug this recipe out of a colleague's thesis..
I havent tried it yet though...
20 mM Tricine
1.07 mM (MgCO3)4Mg(OH)2.5H2O
2.67 mM MgSO4
0.1 mM EDTA
33.3 mM DTT
270 uM Coenzyme A
470 uM Luciferin
530 uM ATP
this may be the original recipe and not the proprietry Promega one...
in my experience I couldnt get luciferin to dissolve in water I used a
luciferin potassium salt...
hope this helps
..... Martin Leach Email:leach at bu.edu
_|____ Dept. of Pharmacology Phone: (617) 638-5323
/ o / Boston Univ. School of Med. Fax: (617) 638-4329
_/ |-/__==/ 80 E. Concord St. (L603)
(BULLDOZER) \_ Boston MA 02118 "Not the old underpants
USA on your
My homepage: http://188.8.131.52/Leach.html
p.s. try BioMOO -virtual biology on the internet..
telnet bioinfo.weizmann.ac.il 8888
Biotech companies on the net: http://184.108.40.206/jumper/biolcomm.html
Dominic Voon (dvoon at uniwa.uwa.edu.au) wrote:
: Hi all,
: The Promega Luciferase assay kit that we purchased is running
: out of luciferase substrate (luciferin, CoA and a mysterious
: competitive inhibitor of luciferase), and we don't want to splash out
: big bucks for another. Would someone be so kind as to tell me (and the
: newsgroup !!) how to make my own luciferase assay substrate ? Any
: information will be much appreciated. If possible, send a copy of your
: followup post to me via email at dvoon at cyllene.uwa.edu.au.
: Many thanks in advance :-)