long distance PCR and Tth?

Duncan Clark Duncan at genesys.demon.co.uk
Fri May 19 08:54:49 EST 1995

In article: <3pbp4e$hh5 at nuscc.nus.sg>  mcbtayhn at leonis.nus.sg (Tay Hou Ngee Agnes) writes:
> Hi
> Does anyone have any experience with the different Tth DNA polymerases 
> for long-distance PCR using genomci DNA as template? I 
> was trying out the Human promoterfinder kit from clontech but they had no 
> enzyme in stock so I substituted Tth from promega and have blanks.
> Would appreciate any advice on longdistance PCR esp with genomic dna as 
> template.

Ordinary Tth needs the addition of 1/16 unit Vent or Pfu adding per 2.5u Tth
for long PCR.

My mind's made up. Don't confuse me with the facts!

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