phenolred: effect on transformation of RECs?

Christian Seelos christian.seelos at univie.ac.at
Fri May 19 06:49:22 EST 1995


For the establishment of an estrogen-inducible gene switch
we changed to a culture-medium that is phenolred free. However, 
under these conditions we no longer observe the cooperating activities
of overexpressed myc and ras in transforming primary rat cells.
Does anybody know anything about this effect?
Thanks a lot
Christian

--
Christian Seelos                    eMail: christian.seelos at univie.ac.at
Universitaet Wien                  Tel.:  +43 1 40154 221
Inst. fuer Tumorbiologie          Fax :  +32 1 4060790
Borschkegasse 8a
1090 Wien
Austria



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