PCR denaturation step- length of time?

RMCMAH93 at IRLEARN.UCD.IE RMCMAH93 at IRLEARN.UCD.IE
Mon May 22 06:45:28 EST 1995


 
I routinely perform denaturation steps for 30-45 sec at 95 degrees C.
But surely  it should only be necessary for the DNA to be denatured for
a matter of seconds, say 5? Any views or comments on this would be
appreciated.
Ruth.



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