hroychow at NMSU.EDU
Tue May 23 16:27:51 EST 1995
On 23 May 1995 grgmj at picr.cr.man.ac.uk wrote:
> HI - sorry to raise this subject again. The trouble was I didn't pay much attention to the discussion at the time and now a colleague is in trouble with his
> PAGE turning into a mosiac. What are the suggestions for drying high
> percentage gels? Fixing protocols? Use of glycerol/TCA??? These are the two
> I remember but not in too much detail! I would (and he) appreciate the
> Cheers, Michelle
After final destaining:
Soak gel in 5% MeOH/10%Acetic acid for 30 min.
Replace with 10% Acetic Acid and shake for an additional 30 min.
Replace with 3% glycerol in 10% acetic acid and soak (w shaking) for 10
to 15 min. This time is empirical and gels of different % need different
time ranging from just 5 min for 7.5% gel to may be 30 min for a 15% gel.
Gradient gels are difficult if they are cast 1.5mm thick. Best
hassle-free drying happens with gels that are 1mm or less in thick ness,
even gradient gels.
Hiranya S. Roychowdhury
Plant Genetic Engineering Lab.
Box 3GL, NM State Univ.
Las Cruces, NM 88003
Phone: (505) 646-5785
hroychow at nmsu.edu
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