Sterilize E-poration cuvettes ?
hroychow at NMSU.EDU
Tue May 23 16:11:28 EST 1995
On 23 May 1995, Andy Phillips wrote:
> seab at nucleus (Stefan Baeckstroem) wrote:
> > Hello Electroporating people.
> > How do you sterilize the single-use cuvettes from Bio-rad ?
> I soak them in 5% bleach for about 10 minutes, rinse ten times
> in distilled water and store under ethanol. Eventually the chlorine
> will pit the electrodes and reduce efficiency of transformation, but
> the cuvettes seem to be reusable for at least ten times.
A word of caution here: some disposable cuvettes will simply dissolve in
95% ethanol. So may be a 20% or so EtOH should be used if storage of
cuvettes is intended. We had a dry sterilization chamber in one of our
Prof.'s lab that used peroxide cans (?). The plastic stuff could be
sealed in special pourous bags, sterilized that way and stored for future
Hiranya S. Roychowdhury
Plant Genetic Engineering Lab.
Box 3GL, NM State Univ.
Las Cruces, NM 88003
Phone: (505) 646-5785
hroychow at nmsu.edu
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