Fast Hybridization and Ligation, how?

Juergen Sasse (Path) js1 at mole.bio.cam.ac.uk
Tue May 23 14:17:21 EST 1995


Hi,
Ambion has recently produced a new hybridization buffer which facilitates 
the hybridization of RNA/DNA in a matter of minutes. Boehringer has 
introduced a ligation buffer which allows ligations to be performed in 
minutes. I wonder if they use the same kind of principle or chemical to 
achieve this. They both claim that they don't use volume excluders like 
dextran sulfate or PEG. Ambion also claims that they don't use the PERT 
technique where probe and target are concentrated at the organic:aqueous 
interphase. Does anybody know what these buffers contain?
Please let me and others know.
Jurgen




More information about the Methods mailing list