Fast Hybridization and Ligation, how?
Juergen Sasse (Path)
js1 at mole.bio.cam.ac.uk
Tue May 23 14:17:21 EST 1995
Ambion has recently produced a new hybridization buffer which facilitates
the hybridization of RNA/DNA in a matter of minutes. Boehringer has
introduced a ligation buffer which allows ligations to be performed in
minutes. I wonder if they use the same kind of principle or chemical to
achieve this. They both claim that they don't use volume excluders like
dextran sulfate or PEG. Ambion also claims that they don't use the PERT
technique where probe and target are concentrated at the organic:aqueous
interphase. Does anybody know what these buffers contain?
Please let me and others know.
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