Safe dye for PCR?
Robert Horton
horton at biosci.cbs.umn.edu
Tue May 23 17:53:59 EST 1995
Malcolm Moos Jr. (moos at helix.nih.gov) wrote:
: Glen Shearer <gshearer at whale.st.usm.edu> wrote:
: >
: > Anyone know of a dye that will not inhibit PCR?
: >
: >The people who sell the air thermal cycler have pointed out that both
: tartrazine and cresol red do not interfere with PCR; of the two, cresol
: red is more intensely colored.
Reference: Hoppe, B.L., Conti-Tronconi, B.M., and Horton, R.M. Gel-loding
dyes compatible with PCR. BioTechniques 12(5):679-80 (1992).
Bob Horton (Ph.D.!) /\ "Crash programs fail because of the theory that
U. of Minnesota, CBS || with nine women pregnant you get a baby a month"
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