Safe dye for PCR?

Robert Horton horton at biosci.cbs.umn.edu
Tue May 23 17:53:59 EST 1995


Malcolm Moos Jr. (moos at helix.nih.gov) wrote:
: Glen Shearer <gshearer at whale.st.usm.edu> wrote:
: >
: > Anyone know of a dye that will not inhibit PCR?
: > 
: >The people who sell the air thermal cycler have pointed out that both
: tartrazine and cresol red do not interfere with PCR; of the two, cresol
: red is more intensely colored.

Reference: Hoppe, B.L., Conti-Tronconi, B.M., and Horton, R.M. Gel-loding 
dyes compatible with PCR. BioTechniques 12(5):679-80 (1992).

Bob Horton (Ph.D.!)   /\ "Crash programs fail because of the theory that
U. of Minnesota, CBS  || with nine women pregnant you get a baby a month" 
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St. Paul, MN 55108    ^^   horton at biosci.cbs.umn.edu/(612) 624-3790



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