Commercial antibody preparation

kruskal at RASCAL.MED.HARVARD.EDU kruskal at RASCAL.MED.HARVARD.EDU
Tue May 23 11:13:19 EST 1995


In article <3p8bom$goa at cisunix1.dfci.harvard.edu>, "Matthew P. Frosch, MD, PhD" <frosch at dsg.harvard.edu> writes:
>I am soliciting advice about what companies provide good service for
>production of antisera to recombinant proteins.  I have used one
>company (they were cheap) for three different fusion proteins and
>not a single sera (for 5 animals) was useful in Western blotting or
>immunofluorescence.
>
>What services have people liked?
>
>What form did you submit your material in?
>
>What animal did you use (the proteins are all human in origin)?
>
>Thanks in advance (as always).
>
>						Matthew
>
>
I have used Pocono Rabbit Farms for production of bunny antisera to purified
native and recombinant proteins, and they usually do quite well.
717-595-7108

Good luck!
________________________________
Benjamin A. Kruskal, MD, PhD
Division of Infectious Diseases
The Children's Hospital
300 Longwood Ave.
Boston, MA  02115
617-355-5152
617-355-6575 (fax)
kruskal at a1.tch.harvard
________________________________



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