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Western Blots from non-denaturing gels

Dave Lukac Lukac at a1.mscf.upenn.edu
Tue May 23 13:17:42 EST 1995

Does anyone have any hints for transferring proteins from non-
denaturing poly-acrylamide gels to nitro-cellulose?  I have been
transferring multi-protein/DNA complexes from EMSA gels with varying 
success, and I am wondering whether or not a step such as soaking the
shift gel in SDS prior to transfer would aid the transfer.

ANy other hints.  Thank you.


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