Western Blots from non-denaturing gels
Lukac at a1.mscf.upenn.edu
Tue May 23 13:17:42 EST 1995
Does anyone have any hints for transferring proteins from non-
denaturing poly-acrylamide gels to nitro-cellulose? I have been
transferring multi-protein/DNA complexes from EMSA gels with varying
success, and I am wondering whether or not a step such as soaking the
shift gel in SDS prior to transfer would aid the transfer.
ANy other hints. Thank you.
More information about the Methods