Protein purification manual needed

Patrick Grealish kallisti at
Wed May 24 21:43:44 EST 1995

Our lab is currently starting some protein purification and we are
interested in acquiring a GOOD manual.
We are doing the Ni 2+ column purification of a HIS-tagged protein, so the
book would require a good section on this
as well as expression info for bacteria, yeast, mammalian and insect. I
have heard of the Methods in Enzymology book, 
can anyone say if this is a good one or not? TIA

hail eris all hail discordia kallisti
Patrick Grealish
kallisti at

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