band on western where no protein

STRUCTURAL NEUROBIOLOGY LAB laurette at msvax.mssm.edu
Thu May 25 11:00:26 EST 1995


Dear Methodologists-
I have run westerns for a while and in the last month
have a band 60-70 kD where there is no sample.
i wear gloves, block with milk and BSA [no change
from before] overnight at 4C and use components
like 2nd antibody from the vectastain kit [and DAB] to stain.

please help, my thesis is due soon!!!!

thanks,

laurette



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