Sterilize E-poration cuvettes ?
szcooley at dale.ucdavis.edu
Thu May 25 10:25:04 EST 1995
Stefan Baeckstroem (seab at nucleus) wrote:
: Hello Electroporating people.
: How do you sterilize the single-use cuvettes from Bio-rad ?
: I don't want to throw the cuvettes after one Zapp and I
: wonder if you have some great method for this.
: I guess that you first rinse them with water or something
: directly after the Zapp and then you use ethanol to sterilize with,
: and then letting them dry, but don't you get a layer of dead cells
: after a short while on the aluminium ?
: Stefan Backstrom
: seab at nucleus.ibg.uu.se
We simply wash with water, incubate 1 hr in 30% HNO3, wash with water,
incubate at least overnight in 95% EtOH. Later, Mike Cooley
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