Q:PCR amplif. problems w/ secondary structure

AShaw5 ashaw5 at aol.com
Thu May 25 16:50:14 EST 1995

I have found that denaturing the DNA really helps on troublesome PCR
reactions that are due to secondary structure.  I use the same protocol
that I would use t prepare a plasmid template for sequencing.  Good luck.
Andrey Shaw

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