Plasmids in E.coli SURE

"Alexander Kraev" bckraev at aeolus.ethz.ch bckraev at wawona
Thu May 25 12:47:11 EST 1995


Dear netters,
I have heard somewhere that "plasmid replication in E.coli SURE is
erratic", but couldn't find this source. Since we have finally
succeeded to clone a promoter fragment using only this strain
(no connection to Stratagene), I would appreciate any specifics
from those having such experiences. My own experience with this
strain is limited to its use with M13 vectors.
Cheers,
*************************************************************************
Alexander Kraev, Ph.D.                 Internet: bckraev at aeolus.ethz.ch
Lab. of Biochemistry III               Phone: 0041-1-632-31-47
Swiss Federal Institute of Technology  FAX:   0041-1-632-12-13
Universitaetstr.16
CH-8092 Zurich
"Some ideas are obscure not because they are complex, but because they 
 are excluded from our circle of comprehension" - Kozma Prutkov



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