Sterilize E-poration cuvettes ?
shin at biosci.cbs.umn.edu
Fri May 26 05:03:07 EST 1995
Stefan Baeckstroem (seab at nucleus) wrote:
: Hello Electroporating people.
: How do you sterilize the single-use cuvettes from Bio-rad ?
: I don't want to throw the cuvettes after one Zapp and I
: wonder if you have some great method for this.
: I guess that you first rinse them with water or something
: directly after the Zapp and then you use ethanol to sterilize with,
: and then letting them dry, but don't you get a layer of dead cells
: after a short while on the aluminium ?
I've used this protocol on one cuvette I had for at least 3 years
(hundreds of transformations E. coli and budding yeast) without any problem.
I checked for cross-contamination by using a Kan resistant E. coli
followed immediately by washing and then transforming a Kan sensitive E.
coli and found in most cases (5/5 that I tested) did not give me any Kan
resistant bacteria. However, for yeast 70% Ethanol rinse did not kill
all the cells for immediate use. If you left the cleaned and ethanol
washed cuvettes for at least a overnight drying in your desk drawer, no
contamination by the yeast from the previous zap.
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