HELP! Genomic DNA not cutting!
rcaspi at ucsd.edu
Fri May 26 16:43:37 EST 1995
In article <S.Rowland-1505952146120001 at 18.104.22.168>,
S.Rowland at auckland.ac.nz says...
>In article <1995May11.191903.7840 at mcrcr6>,
galinm01 at mcrcr6.med.nyu.edu wrote
>> Seeking suggestions on "how to" "clean-up" genomic DNA that
>> refuses to digest with restriction enzymes.
... how to fix this problem by dialysis.
Well, I have had similar problem with a specific strain of bacteria, and in that
case, dialysis did NOT help! I beleieve that I had a problem with
polysaccharides or carbohydrates that would not separate even during
dialysis. What I found to work great, are the new columns of Qiagen,which
remove polysaccharides. What used to take 300 ml of culture and 15(!)
phenol-chloroform extractions, followed by dialysis, is now accomplished with
5 ml in less than one day (not including resuspension of DNA) and the DNA is
I do not own Qiagen (unfortunately) or associated with them by any means.
Hope it helps
Scripps Institution of Oceanography
La Jolla CA 92093-0202
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