Protein purification manual needed

To: Nikolai troianovsk_s at MSDISK.WUSTL.EDU
Fri May 26 15:01:06 EST 1995

>From: kallisti at (Patrick Grealish)
>Subject: Protein purification manual needed
>Date: Wed, 24 May 1995 21:43:44 -0500
>Our lab is currently starting some protein purification and we are
>interested in acquiring a GOOD manual.
>We are doing the Ni 2+ column purification of a HIS-tagged protein, so the
>book would require a good section on this
>as well as expression info for bacteria, yeast, mammalian and insect. I
>have heard of the Methods in Enzymology book, 
>can anyone say if this is a good one or not? TIA

Hi, Patrick

In the begining of your serach for the manual you may look through my
WWW_Page ( I have some info concerning purification
of rec.protein on Ni-NTA agarose (from QIAGEN User`s Manual).

good luck.

email:nikolaic at

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