Protein purification manual needed

[To: Nikolai]

>From: kallisti at merle.acns.nwu.edu (Patrick Grealish)
>
>Subject: Protein purification manual needed
>
>Date: Wed, 24 May 1995 21:43:44 -0500
>
>Our lab is currently starting some protein purification and we are
>interested in acquiring a GOOD manual.
>We are doing the Ni 2+ column purification of a HIS-tagged protein, so the
>book would require a good section on this
>as well as expression info for bacteria, yeast, mammalian and insect. I
>have heard of the Methods in Enzymology book, 
>can anyone say if this is a good one or not? TIA


Hi, Patrick

In the begining of your serach for the manual you may look through my
WWW_Page (http://128.252.119.253). I have some info concerning purification
of rec.protein on Ni-NTA agarose (from QIAGEN User`s Manual).

good luck.

Nikolai
email:nikolaic at visar.wustl.edu
3wP:http://128.252.119.253