SDS-PAGE method for small proteins/peptides

Philp Robin John mcbrp at
Fri May 26 23:22:19 EST 1995

James Davidson (james at wrote:
: Can anyone help me with a good protocol for separating small proteins in the 
: range 1 to 10 kDa using SDS-PAGE?


One such method we use for the separation of small proteins or in our 
case CNBr fragments is the Tris-Tricine method of H. Schagger and G. von 
Jagow. The reference is:
Anal. Biochem (1987) 166, 368-379.

Should separate down to 1kDa. Hope it helps.

Robin J. Philp
Institute of Molecular and Cell Biology
Singapore, 0511.

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