SDS-PAGE method for small proteins/peptides
Philp Robin John
mcbrp at leonis.nus.sg
Fri May 26 23:22:19 EST 1995
James Davidson (james at chempath.uct.ac.za) wrote:
: Can anyone help me with a good protocol for separating small proteins in the
: range 1 to 10 kDa using SDS-PAGE?
One such method we use for the separation of small proteins or in our
case CNBr fragments is the Tris-Tricine method of H. Schagger and G. von
Jagow. The reference is:
Anal. Biochem (1987) 166, 368-379.
Should separate down to 1kDa. Hope it helps.
Robin J. Philp
Institute of Molecular and Cell Biology
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