lambda-bank screening for proteases
MARKUS at scorpio.tng.oche.de
Fri May 26 10:13:00 EST 1995
I'm trying to "fish" protease-genes out of an lambda-bank made from
genomic DNA with inserts from roughly 2-7kb, utilizing Stratagene's kit.
The phage infects E.coli Blue MRF and can be excised with an helper phage
in order to clone a selected insert or the entire bank in E.coli XLOLR.
I can measure proteolytic activity in my culture-supernatant, but I don't
have any sequence-information of the extracellular protease(s), so I can't
use a probe for hybridisation-reactions.
On the other hand I don't want to purify and sequence the protein.
May be the proteases are expressed in their inactive and unexported form
I'd appreciate if I can get any helpful hints !
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