troianovsk_s at MSDISK.WUSTL.EDU
Sun May 28 00:33:56 EST 1995
>Description: Theoretical pI's
>I'm in the process of expressing a protein with about 200 amino acids,
>and my cells are making something that looks like the right thing, but it
>would be nice to verify that. I've just been reading a paper where
>somebody calculates the theoretical pI of his protein and then shows that
>his expression product moves as predicted in 2d electrophoresis.
>Unfortunately he doesn't explain how he works out the value accurately.
>Nobody here can tell me how to calculate a theoretical pI from an amino
>acid sequence except very roughly, so if anybody knows how, or could
>point me to a reference I would be very grateful,
>Thanks a lot,
if you have access to VAX/VMS server with GCG programm installed on it, you
could easy calculate Isoelectric point of a protein based on it aa seq.
Brief summary of the GCG commands you may find at
More information about the Methods