help: RNA extraction from Xenopus embryos

sa95078 sa95078 at odin.mda.uth.tmc.edu
Tue May 30 11:19:23 EST 1995


I tried to use RANzol B from Biotecx to isolate total RNAs from Xenopus
embryos. I followed the protocal come with the product.  I homogenized
the embryos in RNAzol B solution, extracted it with 1/10 of chloroform,
and precipitated RNA in the aqueous phase with same volume of
isopropanol.  I found a whole lot of white precipitate (solid, looked
like salt) that ware hard to be disolved again (even at 60¡C). 

Hope to hear some peoples' opinions.



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