Andy.Phillips at bbsrc.ac.uk
Tue May 30 07:18:29 EST 1995
<ACGMS at CUNYVM.CUNY.EDU> wrote:
> Dear netters:
> Any suggestions why 'rescue' doesn't work?
When I had problems with Zap rescue a few years ago, I finally
tracked the cause down to the cells I was using - I was using XLIB
from a plate that was 2-3 weeks old. Because you need the pilus for
infection with the helper phage, it's essential to select for the
presence of the F plasmid. When I re-plated the cells on Tet, the
rescue worked perfectly. This works for XLIB, not sure about SOLR.
Andy Phillips, IACR-LARS
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