How to decontaminate a DNA-contaminate pipettor?

Alberto Carboni carboni at imiucca.csi.unimi.it
Tue May 30 02:18:18 EST 1995


Dear Bob,

here's a simple method to eliminate DNA from your pipettors.

This method cames from: "PCR protocols" edited by Innis et al. 1990, Academic Press.

Soak the barrel of a pipetman in a beaker containing 0.25N HCl for 30 minutes, repeat with 0.5 N
NaOH for 30 minutes, rinse well with distilled water and dry in a oven for 20 minutes at 60°C.


Good luck,


Alberto Carboni


PhD std.
BIO-DEPT.
Univ. of Milan




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