prot.dialysis ppt

Denni Schnapp ds4 at st-andrews.ac.uk
Wed May 31 08:44:23 EST 1995


Dear netters,

there has been a post recently about proteins precipitating/denaturing
during dialysis. I have a similar frustrating problem with dialysis of
total cell proteins from bloodcells of the shore crab (C. maenas). I
first thought the pH of the dialysis buffer was the problem (glycine
at pH 8.5 to bring sample pH up for ion exchange chromatography) but
the problem persists at neutral pH. I dialyze overnight at 4oC using
membranes with 2000 Da NMWC. Does anybody know what could cause my
proteins to denature?

Thanks for any advise,
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Denni Schnapp
Gatty Marine Laboratory			ds4 at st-and.ac.uk
University of St. Andrews	http://www.st-and.ac.uk/~www_sa/personal/ds4/	
St. Andrews Fife KY16 8LB
Scotland

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