delle at sc2.salk.edu
Wed May 31 23:54:35 EST 1995
obrien at pharm.med.upenn.edu (PJOB) wrote:
>Is there a FAQ regarding the Expressed Sequence Tag database and the clones
yes, I don't know if it is really a FAQ but anyway it explain everything
about EST ... but I don't remember where it is .. sorry .. take a look to
from where you should be able to find it ...
> If not, can anyone tell me if the sequences in the database represent the
>entire insert of a given clone, or just a single sequencing run from one of
>the two universal primers that flank the insert?
At least for arabidopsis, the sequence represent a single run from one
(usually the 5') site of the cDNA ....
I got some clones ranging from 500 to 1400 bp ...
hope this help
Massimo Delledonne, PhD
permanent address: Istituto di Genetica Vegetale
Universita' Cattolica S.Cuore, 29100 Piacenza ITALY
Ph. (+39) 523-599209 Fax (+39) 523-599283
internet: delle at pc.unicatt.it
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