Methods May 1995 Archives by author

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Starting: Mon May 1 00:41:23 EST 1995
Ending: Wed May 31 23:54:35 EST 1995
Messages: 1246

Concentration of proteins in dialysis "Debra Sellers ", CHE
260/280 OD "Debra Sellers ", CHE
Email address of ATCC "Debra Sellers ", CHE
Pseudomonas libraries "Marianne Leverone ", BIO
Pink DNA Preps Rory O'Brien (Biotech)
Fast Hybridization and Ligation, how? Juergen Sasse (Path)
Gel Documentation Luca Ida Giovanni TOLDO (Ph.D.)
DNA quantification B VISSER * x2818 Plantkunde *
Not1 digestion B VISSER * x2818 Plantkunde *
Unpredictably primed PCR
Unpredictably primed PCR
A cloning problem. Kind souls - please help!
Best KIT for PCR-cloning???
Cracked gels..
platinum wires -
Cross-linking glucose to proteins? -
PE Amplicycle - info wanted R. P. Grant +44 1 865 221018
Crystal viole(n)t staining of cells R. P. Grant +44 1 865 221018
PEG R. P. Grant +44 1 865 221018
Ligation Problems R. P. Grant +44 1 865 221018
Baboon cDNA libraries? 3ARB7 at QUCDN.QueensU.CA
platinum wires 4700gbera at umbsky.cc.umb.edu
mutagenesis question at a1.mscf.upenn.edu
NASBA A.Marchant
Attachment of (strept)avidin to strip support A.Marchant
bluescript 'rescuing' ACGMS at CUNYVM.CUNY.EDU
OLIGO Software Package for PCR Primer Selection AShaw5
Q:PCR amplif. problems w/ secondary structure AShaw5
Help! Nonisotopic In situ mRNA: frozen sections. J S Abrams
Encyclopedia/Manual of Protocols J S Abrams
Phage Display System Bernard Abrenica
IMPORTANT INFO FROM NSF ON FUTURE OF INTERNET BIOSCI Administrator
UNSUBSCRIBING, BIOSCI ARCHIVES, ADDRESS DATABASE & BIOSCI FAQ BIOSCI Administrator
IMPORTANT - BIOSCI U.S. Site is moving! (sometime in June) BIOSCI Administrator
cohesive and blunt ligation protocols Claude Aflalo
non-isotopic PCR primers? Tay Hou Ngee Agnes
long distance PCR and Tth? Tay Hou Ngee Agnes
Re BRL 5'RACE kit Tay Hou Ngee Agnes
(Long-Distance) PCR with genomic DNA Tay Hou Ngee Agnes
p53 Western: 35S, Non denaturing, or IP???????? David Aldridge
Pink DNA Preps Malboobi M Ali
q Danny Altshuler
co-immunoprecipitation problem Danny Altshuler
TAP-1 Antibodies Luis M. Alvarez
5'-3' mutagenesis kit Stephen Amato
Metylation-free strain ? Stephen Amato
PCR question Eric C. Anderson
5 prime RACE HELP Eric C. Anderson
5' to 3' exonuclease Eric C. Anderson
HELP! Bacterial strains for PCR cloning Eric C. Anderson
dilution of oligonucleotides Eric C. Anderson
Touchdown PCR Eric C. Anderson
His-rich E.coli Proteins Eric C. Anderson
split bionet.molbio.methds-reagnts? Eric C. Anderson
Western Blotting: What makes it take a long time? Eric C. Anderson
Ligation Problems Eric C. Anderson
mutagenesis question Eric C. Anderson
Sterilize E-poration cuvettes ? Eric C. Anderson
WWW Page for Anti-viral Antibodies Roger Anderson
Long Range DS Sequencing Roger Anderson
Probing lambda libraries with oligonucleotides Roger Anderson
DrdII restriction enzyme Roger Anderson
Probing phage libraries II: Choice of filters Roger Anderson
Genomic library of Maize Roger Anderson
pharmacia e-mail Roger Anderson
Problems with Nae I... Roger Anderson
what is the email of clonetech Roger Anderson
Need X. laevis cDNA libraries Roger Anderson
Antibodies against Vertebrate HOX proteins Tom Anderson
DMSO for tissue culture use Andy
refolding denatured proteins Bruce Appel
Mac program for making banner for poster? Tracy Aquilla
multi color detection kit for DNA Rhodora Argonza
PCR question Ahmet Arman
DNA PREP FOR BACULOVIRUS SYSTEM Curt Ashendel
ECL detection kit Curt Ashendel
What's pKONeo? Curt Ashendel
pipetman calibration Curt Ashendel
Microinjections - hints? Graham Atherton
Help! Need pT7-7 sequence Juan J. Aviles
protein kinase G BIOSCIPA at IPV85.UNIPV.IT
source of Streptolydigin BOCHENTH at CCVAX.SINICA.EDU.TW
MAR/SAR Positive Control! Charolyn Babichuk
Green Fluorescence Protein Reporter Gene Stefan Baeckstroem
Problems with Nae I... Stefan Baeckstroem
Metylation-free strain ? Stefan Baeckstroem
Sterilize E-poration cuvettes ? Stefan Baeckstroem
Where can I get vector pAMP? J.J. Baker
(Long-Distance) PCR with genomic DNA Dr David E Barton
PureGene DNA Extraction Kit Dr David E Barton
PureGene DNA Extraction Kit Dr David E Barton
DNA labeling alternatives Dr David E Barton
PCR labelling of probes Dr David E Barton
Expanded trinucleotide controls Dr David E Barton
"Padlock" Probes Dr David E Barton
IFN-alpha, IFN-beta ELISA-kits ? L. Bastian
******* Short Courses in Molecular Biology ********** L. Bastian
Cheap oligos--SUMMARY L. Bastian
anti phos-serine abs? L. Bastian
PCR errors:How many really? L. Bastian
His-Tag:Single column prep? Dr. Robert Bateman
Maximum plasmid insert size? Uwe Baumert
could protein from dried gel be sequenced? Michael Bausher
5 prime RACE HELP Anne Bazanowski
******* Short Courses in Molecular Biology ********** Fritz Bech
Sequencing high G + C DNA Wendy Bedale
ECL detection kit Wendy Bedale
Proposal for New News Group on Recombination! Wendy Bedale
Human Tumor Cloning Assay Brett Beitzel
LCR and Isothermic Amp. Brett Beitzel
RNase mapping of transcripts Bill Belknap
MUCH CHEAPER good platinum wire Mark Q. Benedict
How MUCH Cheaper Platinum Wire? Mark Q. Benedict
StuI and HincII sites M K Bennett
wanted: clostridium botulinum DNA Randal W Berg
PCR products cloning? Roger Besançon
PBS Gordon Betts
Short course in molbio techniques Gordon Betts
protein extraction from algae Johan Bierkens
Need address of Branson Inc.! Inst. for Biochemistry
Genomic library of Maize Vasos Panagiotopoulos +1-212-287-8087 Bioengineer-Financier
DNA/RNA Isolation Methods and Kits Bioinfo
clipart of mol biol techniques Bioinfo
Help:Company details Bioinfo
EtBr degradation. David F. Bishop
Storage of SDS/PAGE gels - a question Samuel C. Blackman
(none) Yves Blais
DNA purification Jean-Louis Charles Blouin
Human islet cDNA library?? John E. Blume
RNA from fixed tissues. Bob
Best Source for anti-myc and anti-b-gal antibodies Cindy Bolognese
Sequencing high G + C DNA William Bonner
Gas Measurement Cassim Boorany
PCR denaturation step- length of time? Chris Boyd
Fi-X174 DNA amplification, help need! Chris Boyd
Looking for expression vector Jeffrey D. Bray
PCR cycle sequencing Melanie Brazil
Oligonucleotides for Northerns Peter Brophy
Help! Vector Sequence Wanted Mr. A. Brown
intron-exon novice: minimum exon size?? Dan Burgess
Concentration of proteins in dialysis Jonathan Patrick Burke
Email address of ATCC CHAUDH45 at CCVAX.MMC.EDU
normal breast cell line derived from African female CHAUDH45 at CCVAX.MMC.EDU
Grow up of plasmid with large insert CHENJF at HELIX.MGH.HARVARD.EDU
TopPred II 1.2, a new update Manuel .G CLAROS
MitoProt 1.beta a NEW application for mitochondrial proteins Manuel .G CLAROS
PCR : Smearing problems Jo Caine
Q:PCR amplif. problems w/ secondary structure Dr. Fernando Gonzalez Candelas
Binary vector pPCV002 map Alberto Carboni
How to decontaminate a DNA-contaminate pipettor? Alberto Carboni
Jurkat cell line library? M. Carlton
Help - diagnostic PCR lab layout Jeremy Carson
Recording/quantitating electrophoresis gels Tim Carter
Re; WWW Address of CDA Graham Casey
A cloning problem. Kind souls - please help! Ron Caspi
HELP! Genomic DNA not cutting! Ron Caspi
Mac program for making banner for poster? Ron Caspi
heat-shock Ab & probe Diogo Cavaco
HELP ssDNA in agaorse gels Celika
Ambion HybSpeed RNase protection assay kit Daniel Chagnovich
RNAse assay kit Daniel Chagnovich
immunoglobulin sequence database Alex Chang
immunoglobulin sequence database Alex Chang
Long PCR advice needed Jon Chappel
PCR : Smearing problems Jon Chappel
taq isolation Tom Chappell
PCR errors:How many really? Tom Chappell
Post-doc position announcement Kathy Cheah
Yeast as an alternative expression system Sin Wun Chey
Zebrafish ß-actin cDNA fragment Leung Kwan Chi
Sensitivities of prehyb and hybridization solns. Jaishree Muppala Chittoor
Q- Pipettor Calibration Gary Chrebet
Q- Pipettor Calibration Gary Chrebet
Help:Company details Robert Church
Help:Company details Robert Church
Help:Company details Robert Church
HELP! Exo III Dr I.M. Clark
Q: PCR not working Duncan Clark
taq isolation Duncan Clark
taq isolation Duncan Clark
DMSO containing buffers Duncan Clark
taq isolation Duncan Clark
DrdII restriction enzyme Duncan Clark
long distance PCR and Tth? Duncan Clark
Metylation-free strain ? Duncan Clark
Tth pol RT/PCR Duncan Clark
Fi-X174 DNA amplification, help need! Duncan Clark
In vitro transc/transl of maize clones Sonya Clark
Help - diagnostic PCR lab layout Dr. J.P. Clewley
Green Fluorescent Protein (GFP): FACS? Cocea
HELP! Exo III Cocea
Insight into publishing... Jeff Cohlberg
260/280 ratio James T. Colston
Use of Stratagene's LacSwitch Expression System John A. Cook
HELP: cleaning DNA after ligation Michael Cooley
HELP! Bacterial strains for PCR cloning Michael Cooley
DNA from Antirrhinum Michael Cooley
DNA from Monkey Flower (Mimulus) Michael Cooley
About arbitrary primers in differential display Michael Cooley
Sterilize E-poration cuvettes ? Michael Cooley
isolation of RNA from Cardiac Fibroblasts or Myocytes John Cooper
Plasmid drawing program Ron Croy
cohesive and blunt ligation protocols Joaquim Culi
RNazol/lung RNA degradation Tim Cullingford
Microdensitometry of enzymes in single cells Jim Cummins
Colloidal Coomassie Blue Staining Trevor M. D'Souza
pcr cloning artefacts DOCON at ollamh.ucd.ie
help with lambda phage maxi preps! Mr V. Schoenfeld DOM
Stability of tetracycline R Daines
E. coli in microtiter plates R Daines
Genomic DNA Resource Ambiocom - Richard Dana
Call for Papers - DNA Meeting Ambiocom - Richard Dana
mutagenesis question Hong Dang
clipart of mol biol techniques Davepmb
Maximum plasmid insert size? David L. Haviland, Ph.D.
intron-exon novice: minimum exon size?? David L. Haviland, Ph.D.
intron-exon novice: minimum exon size?? David L. Haviland, Ph.D.
Melt 87 Software DavidM7643
Sequencing high G + C DNA DavidM7643
endonuclease Dr Avri Davidoff
SDS-PAGE method for small proteins/peptides James Davidson
Two-hybrid problem...possibly Simon Dawson
cohesive and blunt ligation protocols Simon Dawson
cohesive and blunt ligation protocols Simon Dawson
BrDu Staining Peter Dearden
Problem of the Rearranging Plasmid! Graham Dellaire
Problem of the Rearranging Plasmid! Graham Dellaire
supercoiled DNA and transfection Graham Dellaire
Maximum Insert Size..... Graham Dellaire
Recombining Plasmid Solution!!! Graham Dellaire
Cohesive Blunt end. ligation Graham Dellaire
PCR probes Graham Dellaire
Retro Viral Gene Transfer Graham Dellaire
LOW YIELD of mRNA BY THE USE OF MAGNETIC PARTICLES Massimo Delledonne
Help - cloning into dephosphorylated plasmid vector Massimo Delledonne
Ligation Problems Massimo Delledonne
EST clones Massimo Delledonne
albino mutant on C57Bl/6 background Dr. P. Denny
LOOKING FOR MEMBRANE SPANNING REGIONS Catherine Deon
nonradioactive rflp Edmund Lee Jon Deoon
galactose/galactose oxidase assay information needed M. Deshpande
5 prime RACE HELP Gill Diamond
rt-pcr question Brad A Didion
Techne-GeneE Brad A Didion
d-elutip for genomic DNA Virginia Po Dilegge
help! d-elutip columns Virginia Po Dilegge
PvuI error in Maniatis map of lambda gt10? John Dixon
DraI cut single stranded DNA? John Dixon
Bone biopsies Ole Dj|seland
test Andrew Dodd
Help all you primer extension experts Colin Dolphin
help for transfection! Qunfeng Dong
Help on plant voucher DNA extraction Marcelo C. Dornelas
d/NTP's from DNA - Chemical? Tim Downing
Strainbook Software?? Harold Drabkin
ECL detection kit Harold Drabkin
TA vector rearrangement Yun-Long Du
Temperature-sensitive mutants from random mutatagenesis DukeofHT
HELP! Genomic PCR from plant callus Steve Dunn
lux gene for DNA marking E.T.M
CHEAP X-GAL ANYWHERE? E.T.M
Safe dye for PCR? E.T.M
Source of 24"x30" HEPA filters E.T.M
coli promoter probe JUST ERIC
fractionation of CHO cell membranes Thomas Earnest
How to add an A with Taq Robert Edwards
HELP! Sequence required:pAlter-Ex2. Jan Eggermont
Monoclonal Ab against HA flu epitope Jan Eggermont
(no subject) Klaus Eimert
request: inverse pcr protocol Klaus Eimert
split bionet.molbio.methds-reagnts? Tedd Elich
Email address of ATCC Osman Elmaari
insertion of DNA into E. coli chromosome David A. Elsemore
dilution of oligonucleotides Marion D. Emerson
Host for ss DNA from pBC Endoh, Daiji
Secreted CAT? James Engert
mail Kei-ichi Enjyoji
Sterilize E-poration cuvettes ? Shin Enomoto
LexA antibody Shin Enomoto
Immunoaffinity Subtraction Christer Ericsson
5' to 3' exonuclease Ramesh Errabolu
minimal promoter Ramesh Errabolu
eukaryotic transfections Ramesh Errabolu
Looking for gas monitor Erwan.Corre
E. Coli strain carrying T7 RNA pol ? Thomas Esch
His-Tag:Single column prep? Hernan Espinoza
His-Tag:Single column prep? Hernan Espinoza
E.Coli prep Glibert Fabrice
DNA Precipitating in Sequencing Gel Dr M Fahmy
GFP request Fan
Commercial Ab to Human PDI Darren Fast
HELP needed in western! Darren Fast
quantitative immunoassays Fiberman
pipetman calibration Fiberman
mutagenesis question Fiberman
Luciferase Andrew Fidler
Help: starting RNase protection assays Karl Fischer
anti phos-serine abs? Karl Fischer
ssDNA purification Flanegan
Glutathione-S-Transferase-theta?? Stephen Fletcher
cDNA synthesis problems Barry Flinn
ATPase Dr. W. Flintoff
PCR question Brian Foley
In vitro translation of truncated templates Mick Foley
DIG oligo 5'-end labeling Tracey Forster
PBS John Fox
N terminal amino acid sequencing John E. Fox
could protein from dried gel be sequenced? John E. Fox
Two-hybrid system with lumenal proteins David Franklin
Sequencing high G + C DNA Sherri Fraser
IN SITU PCR Marion Freistadt
New Dedicated Bio-tech/science/chem BBS Suicidal Freshman
New Dedicated Bio-tech/science/chem BBS Suicidal Freshman
New Dedicated Bio-tech/science/chem BBS Suicidal Freshman
Immunoaffinity subtraction P. Friedl
CO2 incubators - your choice? P. Friedl
(no subject) Matthew Frosch
LOOKING FOR MEMBRANE SPANNING REGIONS Matthew Frosch
Automated Sequencer G
Air Control, Inc. Victor Jimenez G.
Source of 24"x30" HEPA filters Victor Jimenez G.
gel doc 1000 uv gel documentation system pc GMIRANDA at IRRI.CGNET.COM
gel doc system? GMIRANDA at IRRI.CGNET.COM
gel do GMIRANDA at IRRI.CGNET.COM
tumour immunology discussion group G Gallagher
Immunoaffinity Subtraction Warren Gallin
Maximum plasmid insert size? Warren Gallin
Shark cartilage Stephen Gately
What's pKONeo? Stephen Gately
Sequencing high G + C DNA Allen Gathman
Denhards Allen Gathman
DNA Meeting, San Diego R. Dana - Green Gene
PCR labelling of probes Charlie Wright Genetics
(none) Maria Luz George
Proposal for New News Group on Recombination! Szatmari George
E-poration of T. congolense Szatmari George
riboprobes for northern blots Michael Gerdes
Windows version of Gel Frag Sizer? Nick Gibson
dilution of oligonucleotides Dr R.V. Giles
Alternatives to Northern???????? Dr R.V. Giles
Doublets after electroelution David Glover
cDNA size separation John Glowacki
Concentration of proteins in dialysis Catherine Godinot
Concentration of proteins in dialysis Catherine Godinot
Sequencing high G + C DNA Steven Goldberg
Probing lambda libraries with oligonucleotides Steven Goldberg
Probing phage libraries II: Choice of filters Steven Goldberg
PCR amplification w/ secondary structure Josh Goldstein
Looking for LexA antibody ! Erica Golemis
PCR amplification w/ secondary structure Fernando Gonzalez
how to respond directly via Netscape? Fernando Gonzalez
HELP! GST fusion protein cleavage Dawn Gray
HELP! GST fusion protein cleavage Dawn Gray
Protein purification manual needed Patrick Grealish
Preparative CNBr cleavage David W. Green
Ligand binding Jonathan R. Greene
HELP! Sequence required:pAlter-Ex2. Mark Griffiths
Sequencing high G + C DNA Barbara Grossmann
Cases of the Rearranging Plasmid and other bothersome Tales!!? Grumbler
Proposal for New News Group on Recombination! Grumbler
Plasmids in E.coli SURE Grumbler
PCR errors:How many really? Grumbler
How to clone PCR products ? Christoph Grunau
two quick questions Xin Guo
help:shuttle vector of PCC6803 H3932Kis at ella.hu
sequencing high G + C DNA HARDIES at THORIN.UTHSCSA.EDU
screening phage library with oligo HARDIES at THORIN.UTHSCSA.EDU
PCR smearing problems HARDIES at THORIN.UTHSCSA.EDU
help on lambda maxi preps HARDIES at THORIN.UTHSCSA.EDU
PEG HARDIES at THORIN.UTHSCSA.EDU
cohesive and blunt end ligation HARDIES at THORIN.UTHSCSA.EDU
ligation problems (w cut PCR product) HARDIES at THORIN.UTHSCSA.EDU
Heop - cloning into dephosphorylated plasmid vector HARDIES at THORIN.UTHSCSA.EDU
PCR errors: How many really? HARDIES at THORIN.UTHSCSA.EDU
Microsatellites LORENZ HAUSER
Rearranging Plasmid Shin-ichiro HIRAGA
Storage of SDS/PAGE gels - a question Kenneth HOWE
PCR primer design Kenneth HOWE
Wizard megapreps Allison Haggarty
DMSO for tissue culture use Allison Haggarty
Help! Plasmid isolation from streptomyces Libby Hamilton
gal4 antibodies Noam Harel
PMSF Solution Noam Harel
In vitro translation of truncated templates Noam Harel
Company address: One-lambda Richard C. Harmon
Source of bone cells Bob Harrison
PMSF solution Ch. Hasse
rat interleukin detection ? Ch. Hasse
Alternatives to Northern???????? Don Haut
Touchdown PCR Don Haut
pT7Blue(R) sequence David Haydon
Theoretical pI's JG. Head
P1 phage prep with Qiagen kit? Amos Heckendorf
Cracked gels.. Amos Heckendorf
Customers of Core DNA Sequencing La Cynthia Helphingstine
Frequently Asked Question (FAQ) list Paul N Hengen
HELP: Ref needed for HOT-start PCR Paul N Hengen
What _is_ a PCR smear? Paul N Hengen
Touchdown PCR Paul N Hengen
ampification of DNA non-methylated at Xba I sites Paul N Hengen
split bionet.molbio.methds-reagnts? Paul N Hengen
platinum wires Paul N Hengen
E. coli in microtiter plates Paul N Hengen
intron-exon novice: minimum exon size?? Paul N Hengen
Safe dye for PCR? Paul N Hengen
His-Tag:Single column prep? Paul N Hengen
DNaseI hypersensitive sites? Paul N Hengen
broad host range vectors Paul N Hengen
HELP! GST fusion protein cleavage Pekka Matti Hilden
PCR question Jody K. Hirsh
In vitro transc/transl of maize clones Jody K. Hirsh
PMSF Solution Jody K. Hirsh
re "in vitro transcr...." Jody K. Hirsh
260/280 OD Jody K. Hirsh
Ligation Problems Sam Hodge
<actin PAb for Westerns Louis Hom
MDM2 specific antibodies??? Charles Honsinger
molbio.methdss-reagnts Andrew Hopkins
sequencing high GC DNA Richard Hopkins
Competitive PCR with internal control Steve Hopkins
anti-TNF Ab EJ Hornby
PCR deletion mutagenesis Robert Horton
Safe dye for PCR? Robert Horton
galactose/galactose oxidase assay information needed Kwang-Jen Hsiao
More GFP info needed I-Jen Huang
GAPDH Neil Hubbard
ANNOUNCEMENT: IRBM practical course: frontiers of protein structure prediction Tim Hubbard
ANNOUNCEMENT: have you a protein to predict? Call for prediction targets Tim Hubbard
Nested PCR Hummel
HELP! My PCR stop working!!!! Robert Hutton
Mysterious plasmid preps Robert Hutton
p65 clone commercially available? Darin Obrien IHG
PureGene Kit - protocol (free!) Darin Obrien IHG
IL-6R signal transducer clone? Darin Obrien IHG
uv-endonuclease HELP IO30470 at MAINE.MAINE.EDU
JURKAT library Immunobiology Research Institute, Siena
WWW Page for Anti-viral Antibodies Goodwin Institute
WWW Page with Antiviral Antibodies Goodwin Institute
pAd10sacB Toshiharu Ishizuka
About arbitrary primers in differential display Toshiharu Ishizuka
RNA Fingerprinting Meeting JFLEMING
Temperature Gradient PCR Cycler JN
PMSF solution JSIGAFOOS
PCR smearing problems JUDITH-C at staff.monash.edu.au
Postdoc Position Hans-Martin Jack
cohesive and blunt ligation protocols Aron Jaffe
refolding denatured proteins Aron B. Jaffe
Help - cloning into dephosphorylated plasmid vector Aron B. Jaffe
computer prog for PEST seq Aron B. Jaffe
gel shifts with labeled protein as a probe?? Aron B. Jaffe
Kits for identification of Osteoblasts? Jamie Love, PhD
cDNA a solution Paul J. Jansen
Probing phage libraries II: Choice of filters Bart Janssen
Mac program for making banner for poster? Bart Janssen
Green Fluorescence Protein Reporter Gene Changcun Jia
nuclear localization signal Changcun Jia
Clontech Marathon kit Jitu
Denhards Per Johansson
Help! pfu DNA polymerase for PCR John P. Aris, Ph.D.
Help! pfu DNA polymerase for PCR John P. Aris, Ph.D.
Help:Company details Philp Robin John
SDS-PAGE method for small proteins/peptides Philp Robin John
Shark cartilage JohnT6020
Long PCR advice needed JohnW86684
PMSF solution Dan Johnson
re "in vitro transcr.poly-A in euk. cells" Jillian Johnston
Purification of GST fusion proteins K Jolley
Employment in biological/biomed.sciences discussion forum-HELP Daniel R. Jones
Reference manager - company email address needed Paul Jones
[Q] Plant cis-acting elements? JongSubLee
[Q] Which kit is the best for plant genomic library? JongSubLee
Q: LCR and isothermic amplification Zophonias O. Jonsson
Quick Denature Plasmid Sequencing Kit Zophonias O. Jonsson
Long Range DS Sequencing Zophonias O. Jonsson
Looking for a Mouse cytotrophoblastic cell line Zophonias O. Jonsson
split bionet.molbio.methds-reagnts? Zophonias O. Jonsson
could protein from dried gel be sequenced? Zophonias O. Jonsson
Ligand binding Rob Jordan
immunopptn Rob Jordan
Pipetters Michael Joseph
Safe dye for PCR? Malcolm Moos Jr.
help: RNA extraction from Xenopus embryos Malcolm Moos Jr.
Cross Hybridization in Northern of Aggrecan Jun-Kyo Suh, Ph.D.
*** IMMUNO TRAINING *** KGEISS%RAVEN at EAGLE.AL.WPAFB.AF.MIL
taq isolation Ron Kagan
taq isolation Ron Kagan
KC8 (mini-preps) Michael Kalchman
??Beta Carotene & Vitamin A?? Ted Kalli
pMSG vector Makoto Kanzaki
Taq Preparation? Ali Karami
Rapid identification of Bacteria? Ali Karami
Looking for LexA antibody ! Thomas Karn
best site directed mutagenesis protocol J. Kaufmann
LA-PCR Keith
PCR : Smearing problems Keith
Help: starting RNase protection assays Merideth Kelley
Molecular Weight etc calculated from chemical formula J. A. Kiernan
Glass milk for 20 bp oligos? Daniel Kim
Shark cartilage Daniel Kim
E. coli in microtiter plates Daniel Kim
platinum wires Daniel Kim
Western Blotting: What makes it take a long time? Daniel Kim
Plasmid drawing program Daniel Kim
Wanted! Monoclonal Ab against N-myc Davis Kim
Immunoaffinity subtraction Dooyeon Kim
Immunoaffinity Subtraction Dooyeon Kim
AH-G 29 Rotor- Need substitute James Kling
Help - cloning into dephosphorylated plasmid vector Bob Kodrzycki
Safe dye for PCR? Eric Kofoid
DNA labeling alternatives Jarkko Kortesmaa
HELP! My PCR stop working!!!! Kevin G. Korth
Strainbook Software?? Kevin G. Korth
OLIGO Software Package for PCR Primer Selection Kevin G. Korth
HELP! Bacterial strains for PCR cloning Kevin G. Korth
strange migration in agarose gels Kevin G. Korth
DNA extraction using microwave??? Kevin G. Korth
DNA purification Kevin G. Korth
Help! Extraction of DNA from formalin-fixed, parrafin-embedded mycobacteria samples for PCR!!! Kevin G. Korth
Wanted-Ab against GAPDH Marcus Korth
Problems with Megaprime labeling Marek Koter
E-poration of T. congolense Jeff Kramer
what is the email of clonetech Andy Kravetz
mutagenesis question Andy Kravetz
Insight into publishing... Andy Kravetz
split bionet.molbio.methds-reagnts? Andy Kravetz
PEG Andy Kravetz
mutagenesis question Andy Kravetz
LKB Superrac 2211 Fraction collector - instruction manual needed Norbert E. Kremer
immunoglobulin sequence database Norbert E. Kremer
More GFP info needed Dubear Kroening
Safe dye for PCR? Dubear Kroening
260/280 OD Dubear Kroening
Plasmid drawing program Dr. Andrew Kropinski
broad host range vectors Dr. Andrew Kropinski
galactose/galactose oxidase assay information needed Arle Kruckeberg
His-tag purification: imidazole quantitation? Arle Kruckeberg
HELP! Hydrophobic/basic protein transfer Arle Kruckeberg
Help: starting RNase protection assays Gary Thomas Kucera
ampification of DNA non-methylated at Xba I sites Stuart Kuhstoss
hIL-2 promoter driving CAT Mr. M. Kuiper
Sequence help Seth W Kullman
RNAseZAP / RNAseAWAY Kusol
HELP!!! Lubrol-PX Paul Kussie
band on western where no protein STRUCTURAL NEUROBIOLOGY LAB
best site directed mutagenesis protocol STRUCTURAL NEUROBIOLOGY LAB
Pink DNA Preps martin LEACH
HELP: Ref needed for HOT-start PCR martin LEACH
intron-exon novice: minimum exon size?? martin LEACH
help for transfection! martin LEACH
Anyone with inverse PCR experience? martin LEACH
help with lambda phage maxi preps! martin LEACH
How to prepare home-made luciferase substrate martin LEACH
test LI, TANG
Help - working in transposon in bacteria. LI, TANG
Equipment for marking animals. LITVAK at VMS.HUJI.AC.IL
Reason for diffs. in mol wgt/ pI LOGAND
PCR cycle sequencing LOGAND
DNA Sequencing +wedge spacers LOGAND
Rf: PCR labelling of probes LOGAND
Rf: Theoretical pI's LOGAND
Rf: Re: A bit of confusion. LOGAND
Rf: Sequence of pUC18 LOGAND
Rf: HELP! Exo III LOGAND
PEG Dean Danner Lab
Cell parody Simerly Lab
How to add an A with Taq Juan Pablo Labrador
PCR primer design Juan Pablo Labrador
RT-PCR Ramkumar Lachumanan
Help - Debug Mammalian Genomic Restriction Digests John Ladasky
HELP! Genomic DNA not cutting! John Ladasky
intron-exon novice: minimum exon size?? John Ladasky
32p agarose gels Morag Lancaster
pZErO-1/Invitrogen-Does it Work Garry P. Larson
RNA Isolation from Arteries Garry P. Larson
Software evaluation Robert Lartey
Looking for macrophages Gordan Lauc
RT/PCR from heparinised blood Zarah Lawal
Where to construct sequence contigs on-line? Martin Leach
New Dedicated Bio-tech/science/chem BBS Martin Leach
Secreted CAT? Martin Leach
Email address of ATCC Martin Leach
anti-NOS antibody Cha Lee
Denhards Rainer Lemke
Sucrose Gradient for DNA fractionation Michael R Leonardo
Sucrose gradients Michael R Leonardo
CO2 incubators - your choice? Victor Levenson
Long-Range DNA-sequencing Victor Levenson
(no subject) Jianming Li
Reference manager - company email address needed Jianming Li
A Question about PCR Edgar Lin
Encyclopedia/Manual of Protocols I-Kai Frank Lin
Encyclopedia/Manual of Protocols I-Kai Frank Lin
terminase & cosmid mapping Jose Casal Lince
terminase & cosmid mapping Jose Casal Lince
T/B cell preps ? Jonas Lindeberg
EtBr carcinogenicity Maxine Lintern
rhodamine? Linton
Sequencher Software Aaron Liston
PMSF solution Jim Litts
manufacturer of restriction enzyme NspH I Tze-Tze Liu
Extraction of SSB Muhammad Lodhi
LOOKING FOR MEMBRANE SPANNING REGIONS Rodrigo Lopez
Paternity testing by PCR Luc.Krols
Commercial antibodies to TBP??? Lopez-Molina Luis
Sequencing high G + C DNA Dave Lukac
Western Blots from non-denaturing gels Dave Lukac
Help! problems with DIG in situ Jukka Luoma
What _is_ a PCR smear? M.J. Lush
Safe dye for PCR? M.J. Lush
Help: Tips for expressing Green Flourecent Protein in Cos cells? Alan Lyons
Problem with making large PCR fragments with TAQPlus Karl Lcking
Anyone with inverse PCR experience? Karl Lcking
help: nested PCR John M.
HELP needed in western! Ted M.
PCR errors:How many really? Ted M.
platinum wires M.DELAVINA at CGNET.COM
platinum wires M.DELAVINA at CGNET.COM
ABI 377 GENOTYPER M.P.Woodland
Help! GST Fusion Protein Expression MAC/se
DNA Sequencing +wedge spacers MAYL at QUCDN.QueensU.CA
mutagenesis question MAYL at QUCDN.QueensU.CA
Attachment of (strept)avidin to strip support MICROPLATE
biotinylated pcr probes MICROPLATE
ColonyPicker 1000 coloniesn per hour MICROPLATE
Help - diagnostic PCR lab layout MICROPLATE
IN SITU PCR MICROPLATE
Glass coated Microplates MICROPLATE
biometra has a new address MICROPLATE
E. coli in microtiter plates MICROPLATE
Pipetters MICROPLATE
split bionet.molbio.methds-reagnts? MICROPLATE
Microplate standardization MICROPLATE
Microtiter Plate/Dish History MICROPLATE
a question in protein purification MICROPLATE
PCR question Jeffrey Ma
Need Glu/Asp-modification at pH7 Marcus Macht
SSCP Equipment - what is best? Jill Maddox
HELP! Bacterial strains for PCR cloning Rafael Maldonado
Metylation-free strain ? Rafael Maldonado
Ligation Problems Rafael Maldonado
Help - cloning into dephosphorylated plasmid vector Rafael Maldonado
Help - cloning into dephosphorylated plasmid vector Rafael Maldonado
EPO-enzyme ? Titta Malinen
Sterilize E-poration cuvettes ? Jon Maltzman
A: PCR not working Susan Mango
OLIGO Software Package for PCR Primer Selection Susan Mango
phage Susan Mango
pcr denaturation step- lenghth of time Susan Mango
split bionet.molbio etc.... Susan Mango
labmda terminase Susan Mango
proteinase inhibitors Zanirato Marco
Will chlorophyll chelate Mg++ in PCR? Jonathan B. Marder
Endothelial Transfection Stewart G Martin
Invitrogen's PCR Optimization Kit? J. Pat Martinez
Invitrogen's PCR Optimization Kit? J. Pat Martinez
Commercial antibody preparation Matthew P. Frosch, MD, PhD
pT7Blue(R) sequence Matthew P. Frosch, MD, PhD
Wanted-Ab against GAPDH David Matthews
Kinase activated by Glycerol Maucuer
What's pKONeo? Marianna Max
total RNA extraction from skin Marianna Max
EST clones Dr S T May
getting DNA out of Mycobacteria Jason McCarthy
Help! Extraction of DNA from formalin-fixed, parrafin-embedded mycobacteria samples for PCR!!! Jason McCarthy
Green Fluorescence protein P. McCray
Controlling A. tumefaciens Alan McHughen
DNA extractin from gel HELP Panhead McNipper
Packaging Extracts Panhead McNipper
PCR products cloning? William Mckendree
gel shifts with labeled protein as a probe?? Fergus Mckenzie
RNA extraction - small sample William Mead
PCR errors: How many really? Jim Meador
Retroviral gene transfer William Meikrantz
DrdII restriction enzyme Meirik at VAX.CS.HSCSYR.EDU
EtBr degradation. Ojar Melefors
in vitro transk. poly A-mRNA in euk. cells Meyer
ECL detection kit Blake Meyers
Rf: HELP! Exo III David Micklem
Help on Rickettsial Genomic Library Construction Mike
Stability of tetracycline Kenneth Minneman
Maximum plasmid insert size? Phillip San Miquel
Maximum plasmid insert size? Phillip San Miquel
Maximum plasmid insert size? Phillip San Miquel
Maximum plasmid insert size? Phillip San Miquel
RNA Extractions from Liver Dr Saleem Mohammed
canine muscle Mr Vid Mohan-Ram
TA cloning Mr Vid Mohan-Ram
WANTED Ab against pig and sheep endothelial cells Thomas Mohr
260/280 OD Barry Moore
band on western where no protein Sean David Moore
Urgent!endoproteinase Lys-C T. Moore
DNA extractin from gel HELP Michael Morales
Ferret Brain cDNA Library Mystery Michael Morales
db for human mitochondrial polymorphims? Jose Moreira
db for human mitochondrial polymorphims? Jose Moreira
http://www.gemini.co.uk/gemini/biopages/ Roger Moreton
RAP-RNA fingerprinting problems Una Morgan
ligation/transformation Shahram Mori
Alternatives to Northern???????? Shahram Mori
PMSF solution Shahram Mori
non-isotopic PCR primers? Shahram Mori
DMSO containing buffers Shahram Mori
About arbitrary primers in differential display Shahram Mori
Theoretical pI's Shahram Mori
HELP needed in western! Shahram Mori
A bit of confusion. Shahram Mori
WANTED! Abs against mitochondria Mike Morris
ABI 377 GENOTYPER Nigel Morrison
intron-exon novice: minimum exon size?? Heidi Moss
ABI sequencing of GC-rich promoter: help! Heidi Moss
taq isolation Anon Y. Mouse
Need address of Branson Inc.! Anon Y. Mouse
TAQ POL ISOLATION Anon Y. Mouse
Touchdown PCR Sandra Munro
PCR labelling of probes Sandra Munro
Commercial supplier of antibodies to Salmonella fimbriae? Pete Muriana
Digoxygenin End labelling Pete Muriana
Sufficient for pub? T. J. Murphy
Double strand sequencing Tony Murray
How to decontaminate a DNA-contaminate pipettor? Charles Murry
Mysterious plasmid preps Peter Myler
oligonucleotide synthesizer Peter Myler
a question in protein purification Daniel Mytelka
Help! problems with DIG in situ User Name
Help! problems with DIG in situ User Name
IFN-alpha, IFN beta ELISA kits User Name
iNOS antibody User Name
riboprobes for northern blots Dr. Kent Nastiuk
Mysterious plasmid preps Richard Near
ampification of DNA non-methylated at Xba I sites Alan Neely
platinum wires Alan Neely
Question about clone manager Rick Neubig
Information about antibiotics? Brad Nicholson
insertion of DNA into E. coli chromosome Brad Nicholson
coli promoter probe Brad Nicholson
Sequencing high G + C DNA Jarmo Niemi
PCR labelling of probes Alan Nighorn
Clontech Marathon kit Allen Nighorn
Storage of SDS/PAGE gels - a question To: Nikolai
Protein purification manual needed To: Nikolai
Theoretical pI's To: Nikolai
A bit of confusion. To: Nikolai
PCR errors:How many really? To: Nikolai
could protein from dried gel be sequenced? J. Norrie
His-Tag:Single column prep Tracy Norris
His-Tag:Single column prep Tracy Norris
His-Tag:Single column prep Tracy Norris
HELP! Exo III Pamela Norton
Secreted CAT? Andy Law Big Nose
ABI 377 GENOTYPER Andy Law Big Nose
ABI 377 GENOTYPER Andy Law Big Nose
pcr cloning blues Rory O'Brien
Help!desaturase activity Operator
INTERACTIVA The virtual laboratory Flavio Ortigao
DNA Precipitating in Sequencing Gel Guy Oshiro
Marfan Syndrome mutation analysis ? Peter Van Osta
taq isolation Anthony Otsuka
His-Tag:Single column prep? Anthony Otsuka
CHEAP X-GAL ANYWHERE? Anthony Otsuka
Information about antibiotics? Jim Owens
Information about antibiotics? Jim Owens
PMSF solution Jim Owens
ssDNA purification Jim Owens
clipart of mol biol techniques Jim Owens
##Trans.Factor program## J Ownbey
Problem with Site-Directed Mutagenesis PAULD at CLONTECH.COM
Posting from M & R newsgroup -Reply PAULD at CLONTECH.COM
Two-Hybrid Problem PAULD at CLONTECH.COM
Mac program for making banner for poster? PJOB
EST clones PJOB
Use of RAPD for somaclonal variation... BANANA & PLANTAIN
Reference manager - company email address needed AL R. PLUMMER
Ferret Brain cDNA Library Mystery ANDREI POPOV
Frameshift mutation in pBS KS ANDREI POPOV
minimum exon size ANDREI POPOV
Maximum high copy plasmid size? ANDREI POPOV
Tth pol RT-PCR ANDREI POPOV
labmda terminase ANDREI POPOV
Inexpensive Power Supplies? PRAKASH at ACD.TUSK.EDU
Idols & ICONS Gurcharan Pahwa
strange migration in agarose gels Garth I. Patterson
Sensitivities of prehyb and hybridization solns. Aaron Pawlyk
HELP!!! Lubrol-PX Francesca Peracchia
protein:DNA uv-xlinking:problems with purified Dan Pereira
His-Tag:Single column prep? Peter
Puromycin James N Petitte
Post-doctoral position in cell cycle/cell division Howard T. Petrie
DNA from Monkey Flower (Mimulus) Rodney Pettway
Green Fluorescence protein James H. Kaysen Ph.D.
DNA Sequencing +wedge spacers Andy Phillips
Sterilize E-poration cuvettes ? Andy Phillips
His-Tag:Single column prep? Andy Phillips
His-Tag:Single column prep? Andy Phillips
Sterilize E-poration cuvettes ? Andy Phillips
bluescript 'rescuing' Andy Phillips
CFTR protein Gerald Pier
Pseudomonas libraries Gerald Pier
Availability of CFTR Gerald Pier
Help! Need cold pCp. Robert Pierce
Ligand screening Piero Pileri Immunobiology Research Institute, Siena
Help! pfu DNA polymerase for PCR T.S. Pillay
Siliconized coverslips Dr. R. Pitera
Where to construct sequence contigs on-line? Philippe Pognonec
biotinylated pcr probes Scott Vande Pol
DIG oligo 5'-end labeling Richard T. Pon
Sequencher Software Sasha Preuss
HELP! GST fusion protein cleavage William P. Prichett
Protein purification manual needed William P. Prichett
Rapid-hyb composition Xose S. Puente
Theoretical pI's RJ. Pugsley
intron-exon novice: minimum exon size?? Rock Pulak
Yeast as an alternative expression system Kevin Pumiglia
Yeast as an alternative expression system Kevin Pumiglia
RNA analysis & PCR methods courses R.Rapley
RNA analysis & PCR methods courses R.Rapley
restrictions on PCR-TRAP RMCMAH93 at IRLEARN.UCD.IE
cycle sequencing RMCMAH93 at IRLEARN.UCD.IE
PCR denaturation step- length of time? RMCMAH93 at IRLEARN.UCD.IE
Paternity testing by PCR ROGER GREEN,MEDICINE,ST.JOHN'S,NF,CAN
Mysterious plasmid preps Colin Rasmussen
taq isolation Colin Rasmussen
Mac program for making banner for poster? Colin Rasmussen
E. coli in microtiter plates Colin Rasmussen
E. coli in microtiter plates Colin Rasmussen
PEG Colin Rasmussen
cohesive and blunt ligation protocols Colin Rasmussen
DNA labeling alternatives Colin Rasmussen
How to decontaminate a DNA-contaminate pipettor? Colin Rasmussen
PEG Colin Rasmussen
band on western where no protein Colin Rasmussen
PEG Colin Rasmussen
Cohesive Blunt end. ligation Colin Rasmussen
to post or not to post ... Colin Rasmussen
DNA labeling alternatives Colin Rasmussen
Purifying DNA onto silica from TBE gels? Raymond Dalgleish, Dept of Genetics
DNA form FORMALIN fixed specimens Guy Reeves
notes on DNA from FORMALIN fixed specimens Guy Reeves
Help! Extraction of DNA from formalin-fixed, parrafin-embedded mycobacteria samples for PCR!!! Guy Reeves
Maximum High Copy Plasmid Size? Brian Reid
Maximum High Copy Plasmid Size? Brian Reid
rat IL6 Dr Schliebs Reinhard
ABI sequencing of GC-rich promoter: help! Udo Reischl
GFP Udo Reischl
Help - cloning into dephosphorylated plasmid vector Nick dos Remedios
Enhance Software- Plasmid Map Enhancer , any users. Mary P. Remington
Split Second DNA Prep Kit B-M Mary P. Remington
RNA extraction from adipose tissue? Mary P. Remington
PCR errors Maido Remm
His-rich E.coli Proteins Richard_Heath
dehydrating bacterial cells? Richard_Heath
Help - working in transposon in bacteria. Richard_Heath
Film for UV gel-images George Riley
260/280 ratio Rimantas.Plaipa at lt.VU.GF
PCR question Julie Roberts
ABI sequencing of GC-rich promoter: help! Bruce Roe
OLIGO Software Package for PCR Primer Selection Dr. Massimo Romani
OLIGO Software Package for PCR Primer Selection Dr. Massimo Romani
Restriction Site Mapping Software Gabriel Romero
co-immunoprecipitation problem David Roof
How can I precipitate down Protamine phosphate? Rory.Morty
HELP! Genomic DNA not cutting! Shelley Rowland
Storage of SDS/PAGE gels - a question Hiranya Roychowdhury
Explanation needed for DNA that "sticks" to wells Hiranya Roychowdhury
taq isolation Hiranya Roychowdhury
platinum wires Hiranya Roychowdhury
RNAseZAP / RNAseAWAY Hiranya Roychowdhury
platinum wires Hiranya Roychowdhury
quantitative immunoassays Hiranya Roychowdhury
DNA Sequencing +wedge spacers Hiranya Roychowdhury
DNA Sequencing +wedge spacers Hiranya Roychowdhury
a question in protein purification Hiranya Roychowdhury
Safe dye for PCR? Hiranya Roychowdhury
260/280 OD Hiranya Roychowdhury
help with lambda phage maxi preps! Hiranya Roychowdhury
enzymes Hiranya Roychowdhury
enzymes Hiranya Roychowdhury
Help with cDNA Hiranya Roychowdhury
Sterilize E-poration cuvettes ? Hiranya Roychowdhury
UV-Cross linking Hiranya Roychowdhury
Cracked gels.. Hiranya Roychowdhury
Western Blots from non-denaturing gels Hiranya Roychowdhury
Help - cloning into dephosphorylated plasmid vector Hiranya Roychowdhury
LKB Superrac 2211 Fraction collector - instruction manual needed Hiranya Roychowdhury
Help - cloning into dephosphorylated plasmid vector Hiranya Roychowdhury
CIPing Hiranya Roychowdhury
Glass milk for 20 bp oligos? Geoffrey Rule
Glass milk for 20 bp oligos? Geoffrey Rule
How to decontaminate a DNA-contaminate pipettor? Robert Rumpf
re "in vitro transcr.poly-A in euk. cells" S.
re "in vitro transcr...." S.
split bionet.molbio.methds-reagnts? Steven L. Sabol
Where can I get NOAM? Jon Sadowaski
RT-PCR Klaus Salger
5' to 3' exonuclease Klaus Salger
(no subject) Klaus Salger
Digoxygenin End labelling Klaus Salger
Alternatives to Northern???????? Klaus Salger
Metylation-free strain ? Klaus Salger
cohesive and blunt ligation protocols Klaus Salger
DNA labeling alternatives Klaus Salger
DNA labeling alternatives Klaus Salger
NF-kappa B family Antibodies Claude Saliou
Sequencing error rate? Mika Salminen
total RNA extraction from skin Leanne Sammels
biometra has a new address Tony Sanchez
Touchdown PCR Dominique Schaller
pharmacia e-mail Denni Schnapp
prot.dialysis ppt Denni Schnapp
a-murCD14-Ab ??? Holger Scholz
PCR question Andrew Schrader
RNA/DNA/PROTEIN FROM SAME SAMPLE?? Andrew Schrader
in vitro transcription of large products, help!!!! Bernhard Schu
Controlling A. tumefaciens Peter L. Schuerman
Sequencing high G + C DNA Daniela Sciaky
Zeocin and background free cloning Daniela Sciaky
Transfection of E1a/ras transformed cells? Christian Seelos
phenolred: effect on transformation of RECs? Christian Seelos
SDS-PAGE method for small proteins/peptides Bart Sefton
(no subject) Bart Sefton
Cracked gels.. Bart Sefton
co-immunoprecipitation problem Bart Sefton
Retroviral gene transfer Bart Sefton
GFP Jeffrey Segall
IFA in yeasts Harpin Serge
Best KIT for PCR-cloning??? Harpin Serge
N terminal amino acid sequencing G Shafer
Clonetech Marathon RACE? Glen Shearer
Cheap oligos--SUMMARY Glen Shearer
Safe dye for PCR? Glen Shearer
Gas measurement Shenaz
Gas flow measurement Shenaz
gas flow measurement Shenaz
gas flow measurement Shenaz
Source for IGFBP's ? A. Sheppard
Source for IGFBP's ? A. Sheppard
EMBL WWW Nucleotide Sequence Submission System Benny Shomer
dilution of oligonucleotides Richard E. Showalter
HELP! Genomic DNA not cutting! Richard E. Showalter
Will chlorophyll chelate Mg++ in PCR? Mark Siddall
Temperature-sensitive mutants from random mutatagenesis Sandy Silverman
Worm in situ hybridization technique wanted J Singer
Sucrose gradients David Singleton
Mysterious plasmid preps Ole Skovgaard
E.Coli prep Ole Skovgaard
DNA Sequencing +wedge spacers Ole Skovgaard
Double strand sequencing Jeff Smiley
Double strand sequencing Jeff Smiley
Help with mouse genomic southerns? Mike Snaith
computer prog for PEST seq Soma
constitutively expressed poato gene Soma
Anyone with inverse PCR experience? Soonmyoung Paik, M.D.
P1 phage prep with Qiagen kit? Soonmyoung Paik, M.D.
DNA extraction using microwave??? Sophie Launey, These Andre Gerard 31 12 96, Ifremer DRV RA, 46.36.30.07
Does anyone have pBRNeo? Felipe N. Soto
Genomic library of Maize Aleksander Sowa
Urgent!endoproteinase Lys-C Anne Spang
PCR cycle sequencing Darren M. Stauth
EtBr degradation. Kaj Stenberg
Mac program for making banner for poster? Stephen R. Lasky, Ph.D.
How to detect DNA mismatches? Eric Stephen
protein based standards for DNA/CE Beth Stewart
streptavidin coated seq plates Beth Stewart
How to detect DNA mismatches? Gregory Stuart
Email address of ATCC Karl A. Suiter
DNA:Glass binding for 100 - 250 bp? Zal Suldan
Problem with making large PCR fragments with TAQPlus Sailesh Surapureddi
cleared lysate prep Sailesh Surapureddi
split bionet.molbio.methds-reagnts? Sailesh Surapureddi
Ligation Problems Sailesh Surapureddi
EtBr Clemens Suter-Crazzolara
Sequence of pUC18 Jan Svensson
In vitro translation of truncated templates NICHOLAS THEODORAKIS
how to respond directly via Netscape? TOURKINE
TECH. SUPPORT REP. WANTED TROPIX at BIOTECHNET.COM
lux gene for DNA marking Paul Tadich
PCR : Smearing problems TedM
Taq isolation TedM
Thermalase Tbr?? Jeffrey Temple
Source for anti HA1 antibody? Nancy Terryn
Monoclonal Ab against HA flu epitope Nancy Terryn
pipetman calibration Tom Thatcher
riboprobes for northern blots Jeff Thornsberry
Test.Please ignore Graham J Threadgill
mtDNA vector anyone???? Gus Trautweiler
In situ PCR of bacteria? Anyone with experience of this? Paul J Travers
full-length COL1A2 cDNA available from ATCC Gerard Tromp
Encyclopedia/Manual of Protocols Rick Tullis
Taq Man Probes Rick Tullis
His-Tag:Single column prep Bradley Turner
LOW YIELD of mRNA BY THE USE OF MAGNETIC PARTICLES Michael J. Tuvin
How can I precipitate down Protamine phosphate? U12201 at uicvm.uic.edu
Glass milk for 20 bp oligos? U12201 at uicvm.uic.edu
Cross-linking glucose to proteins? U12201 at uicvm.uic.edu
ECL detection kit U12201 at uicvm.uic.edu
ECL detection kit U12201 at uicvm.uic.edu
molbio.methdss-reagnts U12201 at uicvm.uic.edu
American Biotechnology Lab U12201 at uicvm.uic.edu
Glass milk for 20 bp oligos? U12201 at uicvm.uic.edu
HELP! Genomic DNA not cutting! U12201 at uicvm.uic.edu
RNAseZAP / RNAseAWAY U12201 at uicvm.uic.edu
Detecting EACA? U12201 at uicvm.uic.edu
Windows version of Gel Frag Sizer? U12201 at uicvm.uic.edu
Enhanced Chemiluminscent HRP susbtrate. U12201 at uicvm.uic.edu
Strainbook Software?? Tim Uphoff
Source for anti HA1 antibody? Virginia Urquidi
Long Range DS Sequencing VICDEF at BIOLOGY.BIOSCI.WAYNE.EDU
HELP REGULATED EXPRESSION CLONING VECTORS Edgar Valencia
Transcriptional terminators Info Edgar Valencia
Quick Denature Plasmid Sequencing Kit Jyrki Valkama
Explanation needed for DNA that "sticks" to wells Richard Varhol
Gel Shift Protocols James Varnum
Kary Mullis, email address? Jason Velick
Prof. Axel Brennicke new address Rafael Perez Vicente
Kinase activated by Glycerol Vinnie
HELP needed in western! Vinnie
How to prepare home-made luciferase substrate Dominic Voon
Sequencing high G + C DNA Karl Voss
taq isolation Stephane Vuilleumier
PMSF solution Stephane Vuilleumier
Ambion HybSpeed RNase protection assay kit Bernard Khoo WCI
DNA PREP FOR BACULOVIRUS SYSTEM DARLA WISE
HELP needed in western! WZhao80850
PCR deletion mutagenesis Fred Wagner
PCR errors:How many really? Warren Wakarchuk
DNA form FORMALIN fixed specimens Fred Waldman
Immunoaffinity Subtraction Amy Walker
looking for assays for theses products of glucose metab. Don A. Walthers
how strong does a cell disrupter needs? Kao-Chung Wang
Safe dye for PCR? Beth Wapelhorst
canine muscle Pearse Ward
Extreme 5' end with RACE? Friedemann Weber
MDM2 specific antibodies??? Nicholas Webster
DAB chemistry as a chromagen Richard G. Wehby
Dissolved oxygen test Tim Welch
SDS-PAGE method for small proteins/peptides David J. Westenberg
Gel QuickPrint Johnny Wharton
Digoxygenin End labelling Wiggy
His-Tag:Single column prep? Iain Wilson
His-Tag:Single column prep? Iain Wilson
His-Tag:Single column prep Iain Wilson
Help! Need pT7-7 sequence Iain Wilson
Binding to plastic=denaturation? Troels Wind
Binding to plastic=denaturation? Troels Wind
His-Tag:Single column prep? Stephan Witte
refolding denatured proteins Stephan Witte
Help: FactorXa Clavage - 0.1 M NaCl required? Stephan Witte
lambda-bank screening for proteases Markus Woischnik
CDM for streptococcus pyogenes Markus Woischnik
Packaging Extracts Julian Wood
EST clones Jim Woodgett
immunopptn R. Woodward
dehydrating bacterial cells? Bradley Workman-Davies
Pharmacia pDR540 tac Promoter Vector Chao I Wu
Anyone know of any good molecular biology books on tape? John Wurzelmann
position opening Herbert Wyckoff
Post-doc position announcement Herbert Wyckoff
position opening Herbert Wyckoff
systematic sequencing Y.L.Dodoo
Membrane spanning.. Akemi Yagi
OLIGO Software Package for PCR Primer Selection Chihiro Yamada
CHEAP X-GAL ANYWHERE? Chihiro Yamada
CHEAP X-GAL ANYWHERE? Chihiro Yamada
[Q]CsCl purification of cosmid NAKAMURA Yasukazu
E. Coli strain carrying T7 RNA pol ? Chris Yates
Help! pfu DNA polymerase for PCR Yi, Xiaoming
Poly-ADP ribose polymerase cDNA ? Yoosik Yoon
Preparing library DNA for expression cloning Ian A. York
Company address: One-lambda Ian A. York
What's pKONeo? Ian A. York
What's pKONeo? Ian A. York
RNAse assay kit Ian A. York
His-Tag:Single column prep? Yurii
Looking for expression vector Yurii
Sp1 Assay column ZOOMmmm
PEG ZOOMmmm
HELP! INSECT DNA EXTRACTION Marco Zanirato
PCR for insect DNA Marco Zanirato
RNase Zap Zeiler, Brian M'bio
CPRG assay Beilin Zhang
cycle sequencing Yi Zhang
Help! pfu DNA polymerase for PCR Yi Zhang
LOW YIELD of mRNA BY THE USE OF MAGNETIC PARTICLES Xin-Hua Zhou
Mysterious plasmid preps Guan Zhu
Ligand binding Frances Hannan Zoology
Probing lambda libraries with oligonucleotides Frances Hannan Zoology
help with lambda phage maxi preps! Frances Hannan Zoology
Colloidal Coomassie Blue Staining Christian Zwieb
Virus extraction ahung at ASRR.ARSUSDA.GOV
Virus extraction ahung at ASRR.ARSUSDA.GOV
request for advice on BAC libraries, etc. aida at CGL.UCSF.EDU
Sequencing high G + C DNA ajeffs at chmeds.ac.nz
PMSF solution arturo.galvani at itner.pharmacia.se
anti CD4? arturo.galvani at itner.pharmacia.se
anti-CD4? arturo.galvani at itner.pharmacia.se
cohesive and blunt ligation protocols austin at camtwh.eric.on.ca
Promega TAQ Bad Batch? baharvey at swansea.ac.uk
Extraction of SSB basse
Sequence help "Alexander Kraev" bckraev at aeolus.ethz.ch
Long Range DS Sequencing "Alexander Kraev" bckraev at aeolus.ethz.ch
PCR cycle sequencing "Alexander Kraev" bckraev at aeolus.ethz.ch
DNA Sequencing +wedge spacers "Alexander Kraev" bckraev at aeolus.ethz.ch
split bionet.molbio.methds-reagnts? "Alexander Kraev" bckraev at aeolus.ethz.ch
Help - cloning into dephosphorylated plasmid vector "Alexander Kraev" bckraev at aeolus.ethz.ch
Plasmids in E.coli SURE "Alexander Kraev" bckraev at aeolus.ethz.ch
to post or not to post ... "Alexander Kraev" bckraev at aeolus.ethz.ch
Transposon for in vitro mammalian gene disruptions benedik at uh.edu
co-immunoprecipitation problem bg2z at musicb.mcgill.ca
PEG bio.tamu.edu
RnaseH digestion? biolc4 at jetson.uh.edu
Pharmacia Spectrophotometer bionet at ncl.ernet.in
Cloning long PCR products bresatec
Detecting EACA? brummer at cc.helsinki.fi
Sequencing high G + C DNA joanna bruno
HELP: cleaning DNA after ligation joanna bruno
p53 Western: 35S, Non denaturing, or IP???????? steven joseph chmura
WEHI-231: p53 status. steven joseph chmura
Water coomassie recipe ?? clement at cvlab.harvard.edu
TRANSFECTING CHONDROCYTES colla at ibcp.fr
Help with mouse genomic southerns? converrl at ucbeh.san.uc.edu
in-gel Klenowing of fragments d.adelson at prospect.anprod.csiro.au
galactose/galactose oxidase assay information needed bipin dalmia
Yeast as an alternative expression system bipin dalmia
Commercial antibodies to TBP??? damania
5 prime RACE HELP abhijit datta
non-radioactive detection detayl at delphi.com
IFN-alpha, IFN-beta ELISA-kits ? m.t. dittmar
PCR Cloning from cDNA libraries docon at midir.ucd.ie
ABI 377 DNA sequencer eckloff at rcf.mayo.edu
Workshop Single Molecules joerg enderlein
galactose/galactose oxidase assay information needed larson eric
best site directed mutagenesis protocol batu erman
protein ppt ing during dialysis batu erman
Size of DNA fbouche at quick.cc.fundp.ac.be
In vitro labelling of plasmid fbouche at quick.cc.fundp.ac.be
SDS-PAGE method for small proteins/peptides ffr.de
Glutathione dependent transcription? How? g1687jkarh at umbsky.cc.umb.edu
HELP! Genomic DNA not cutting! galinm01 at mcrcr6.med.nyu.edu
Sequencing high G + C DNA ronald goto
Cracked gels.. grgmj at picr.cr.man.ac.uk
Fi-X174 DNA amplification, help need! gsbs1018 at UTSPH.SPH.UTH.TMC.EDU
Problem of the Rearranging Plasmid! hardies at thorin.uthscsa.edu
Mysterious plasmid preps hardies at thorin.uthscsa.edu
Polyacrylamide Gel Shrinkage hardies at thorin.uthscsa.edu
test hfakhour
TA vector rearrangement hfakhour
HELP ssDNA in agaorse gels hjs
ssDNA purification hjs
Norther and rRNA ibmi0 at cc.uab.es
Cross-linking glucose to proteins? ingen at mack.rt66.com
UV-Cross linking ingen at rt66.com
MS CONFERENCE Reg. Deadline May 15 irc at III.NET
Antisense Oligo Sequences j.lamb
Canola cDNA library dolman jayne
Ferret Brain cDNA Library Mystery jfh
GAPDH jfh
RT-PCR jm
RT/PCR from heparinised blood jm
Quantitative RT-PCR jm
RNA extraction - small sample jm
promoter for dermal fibroblasts? kamin johnson
Subtraction libraries jr at dna.bio.warwick.ac.uk
incorporating dye labelled nucleotide into PCR product jspencer at blackburn.med.su.oz.au
small bp DNA ladders jspencer at blackburn.med.su.oz.au
Dr. Berndt Schulz-Harder jthrppel at vax1.tcd.ie
In search of cDNA libraries jthrppel at vax1.tcd.ie
taq isolation jurgenson at cobra.uni.edu
Question about clone manager k.d.preuss
Anyonme heared of kasugamycine ? k01a
ampification of DNA non-methylated at Xba I sites kang at msvax.mssm.edu
PCR amplification w/ secondary structure kertesz at micro.biol.ethz.ch
ELISA for TGF-b and PDGF-AA kolpakov at umich.edu
anti-NOS antibody dr. jack komisar
Slide attachment for in situ PCR dr. jack komisar
Plasmid drawing program kooyman at sci.kun.nl
Commercial antibody preparation kruskal at RASCAL.MED.HARVARD.EDU
Glutathione-S-Transferase-theta?? ktgeiss at miavx1.acs.muohio.edu
test post Martin leach
Frameshift mutation in BluescriptKS lidaniel
BluescriptKS and MacConkey plates lidaniel
Binding to plastic=denaturation? littgj at esvx23.es.dupont.com
Paternity testing by PCR lonnie
a question in protein purification yuling luo
E. coli in microtiter plates ted m
PCR denaturation step- length of time? ted m
HELP:c-fms,exon1 marat
cleared lysate prep paolo de marco
DNA labeling alternatives marivonne
Request: Aussie anti mouse CD8 mdcabl at cc.newcastle.edu.au
Southern for methylation millerdl at gunet.georgetown.edu
Training in Biotechniques (fwd) mln
Training in Biotechniques (fwd) mln
Gel Shift and GST fusion protein mmai at kean.ucs.mun.ca
LA-PCR mmg6
HELP: cleaning DNA after ligation mol1 at ifm.mh-hannover.de
help: amplification, polysaccharides monnerot at cgmvax.cgm.cnrs-gif.fr
help: amplification,polysaccharides (complete version) monnerot at cgmvax.cgm.cnrs-gif.fr
Promega TAQ Bad Batch? mpk6
CHEAP X-GAL ANYWHERE? mpk6
ampification of DNA non-methylated at Xba I sites mpk6
HELP: guinea pig cDNA library needed! your name
crystallography position (fwd) (fwd) m.lakshmi narayanan
cheap X-Gal nsaunders at molbiol.ox.ac.uk
GUS antibodies? otello at sc2a.unige.ch
Affinity purifying polyclonal antiserum p-jenner at nimr.mrc.ac.uk
Hot probes using PCR paul.boss at adl.hort.csiro.au
Green Fluorescence protein plancks.res.cei.ohsu at ohsu.edu
Any reference programs on the net? raman913 at uidaho.edu
Polyacrylamide Gel Shrinkage reese at msscc.med.utah.edu
HELP-polyacrylamide gels reese at msscc.med.utah.edu
two-hybrid, detection fusionprotein? reinhilde at lmb1.rug.ac.be
help with lambda phage maxi preps! rlconverse at delphi.com
Crystal viole(n)t staining of cells ryan at mbcf.stjude.org
PCR primer design sa95078
help: RNA extraction from Xenopus embryos sa95078
Encyclopedia/Manual of Protocols sb9e
ligation/transformation schroeder at vzdmza.zdv.uni-mainz.de
help:broad host range vectors schupp at nauvax.ucc.nau.edu
Hi. I am looking for information and experiences with broad host range vectors, schupp at nauvax.ucc.nau.edu
broad host range vectors schupp at nauvax.ucc.nau.edu
E. coli in microtiter plates scolnipa at esvx12.es.dupont.com
PCR errors seppen at amc.uva.nl
Cell-Lines HP13 & RL90 ? Anyone (anti PDI monoclonal hybridomas) sfletche at extro.ucc.su.OZ.AU
supercoiled DNA and transfection shadow
HELP! Bacterial strains for PCR cloning sl6dp at cc.usu.edu
Best KIT for PCR-cloning??? sl6dp at cc.usu.edu
EtBr degradation. smb14 at cornell.edu
Encyclopedia/Manual of Protocols Braudel Maqueira soporte
Rat 5' UT... Braudel Maqueira soporte
Wanted: EBV EBNA-2 oligo-sequences for RT-PCR stephan at lmb1.rug.ac.be
dilution of oligonucleotides cynthia stryker
DMSO containing buffers cynthia stryker
PEG tedm
Quantitation of RNA teferaw at aa.wl.com
Journal Impact Factor uks13mh
Great cDNA Libs varyc.mmcri at OFFICE.MMC.ORG
pMalC2 Ligations varyc.mmcri at mmc.org
Pipetters vboveia at inetnebr.com
Expanded trinucleotide controls beth wapelhorst
HELP! Establishing number of target molecules wick at cris.com
Need X. laevis cDNA libraries chris wilkes
DNA polymerase slippage xpy2 at musicb.mcgill.ca
HELP! Hydrophobic/basic protein transfer lingyu zhang
His-Tag:Single column prep? zinc
[Q]CsCl purification of cosmid zinc
band on western where no protein zinc
Help with cDNA zinetti at acpub.duke.edu

Last message date: Wed May 31 23:54:35 EST 1995
Archived on: Sun Mar 6 20:34:57 EST 2005

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