Colloidal Gold total protein stain
klenchin at macc.wisc.edu
Wed Nov 1 13:12:54 EST 1995
In article <477q8p$102 at mserv1.dl.ac.uk>,
LOGAND <logand at msdos.ensam.inra.fr> wrote:
>Can anyone provide or point to me towards a method to stain total protein
>on western blots (nitrocellulose) after antibody detection of specific
>antigen using alkaline phosphatase ?
AFTER will not work - there is no way to avoid blocking membrane for ab
detection, which means your total protein is all over the blot.
BEFORE is easy. Ponceau S is reversible stain (one drawback - not vry
Stock: 2% solution in 30% TCA, 30% sulfosalicylic acid (store rt).
Staining: dilute 1:10 with water, stain for 10-20 min.
Destaining: water or TBS. Then proceed with blocking for ab staining.
Hope it helps.
/ /\ Dima Klenchin
/ / \
/ / /\ \ klenchin at macc.wisc.edu
/ / /\ \ \ tel. (608)262-4380
/ /_/__\ \ \ FAX (608)262-4570
/________\ \ \
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