Electroporation of HMW DNA??? ;-)

Dima Klenchin klenchin at macc.wisc.edu
Thu Nov 2 12:52:21 EST 1995


In article <47an4l$4oc at oban.cc.ic.ac.uk>,
   Spiros Servos <s.servos at ic.ac.uk> wrote:

>I want to electroporate a series of HMW (ca. 10Kb) recombinant plasmids 
>into E. coli. Does anyone have any advice on how I should best set about 
>doing this?

Check recent NAR (no earlier than Jul), "Methods section". There was a paper on
just exactly that. In brief, 2 mm cuvettes, 2.5 kV, 40 ul, 200 Ohm. 

Good luck. 

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