RNAase digestion of RNA/cDNA hybrid

steve hartson shartson at BMB-FS1.BIOCHEM.OKSTATE.EDU
Thu Nov 2 11:10:13 EST 1995


Simon,
  It sounds like you're describing a (ribo)nuclease protection assay.  I
found the following ref's to be excellent starting points for learning this
technique:

        Kedzierski and Porter (1990) Mol. Brain Res. 7:45-51
        Kedzeirski and Porter (1991) Biotechniques 10:210-214

My experience was consistent with assertations that good results with this
technique *require* empirical optimization.





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