RT-PCR problems

Wolfgang Schechinger diabetes at lrz.uni-muenchen.de
Thu Nov 2 12:19:24 EST 1995

Hi Mark!

I was using the same kit for an RT-PCR. I started with oligo-dT primers and alternatively use 
the random primers, too.
While the oligo-dT yielded only smear in my case, the random primers were working fine!
Maybe you could try that?
Wolfgang Schechinger
Institute for Diabetes Reserch 
Koelner Platz 1
80804 Munich
Phone +49 (89) 30 79 31 24
Fax   +49 (89) 30 81 733
email u7k0201 at sunmail.lrz-muenchen.de

(Standard disclaimer applies)

More information about the Methods mailing list