Hi! I want to electroporate a series of HMW (ca. 10Kb) recombinant plasmids into E. coli. Does anyone have any advice on how I should best set about doing this? In particular what conditions should I use? Please e-mail as well as post any replies as my access to news is not good. TIA Spiros Servos Post-doc in the Dept. of Biochemistry, Imperial College