Electroporation of HMW DNA??? ;-)

Spiros Servos s.servos at ic.ac.uk
Thu Nov 2 10:12:21 EST 1995


Hi!

I want to electroporate a series of HMW (ca. 10Kb) recombinant plasmids 
into E. coli. Does anyone have any advice on how I should best set about 
doing this? In particular what conditions should I use?

Please e-mail as well as post any replies as my access to news is not 
good.


TIA

Spiros Servos
Post-doc in the Dept. of Biochemistry, Imperial College





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