rapd pcr artifactual products

vid mohan-ram k9mhc at rfhsm.ac.uk
Wed Nov 1 19:07:16 EST 1995


'problem 1' : using a single 10mer (random) primer i get pcr products from 
200bp to 3kb with no template dna....the question is how does a single 10mer 
primer create sufficient secondary structures for such amplifications?
has anyone else seen this?....some of these bands ARE seen in rxns using 
genomic dna...

'problem' 2: whats the program of choice when it comes to analyzing rapd pcr 
products to create phylogeny trees etc...and how can i get access to them?

thanks

vid



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