Dear Fellow Scientists,
I am in urgent need of a quick and clean
method for extracting dna from
lymphocytes to be run on a gel.
I previously quantitated my dna
by using 5 pg/cell as the standard
amount, and counting the number of
cells I needed for one microgram.
Upon cleaning these cells in PBS,
then suspending them in an alkaline
solution with SDS, then using an
alkaline loading buffer to load them
in a gel. The problem I seem to be having
is being quantitatively consistent.
Using densitometric analysis, I have discovered
that not all of the DNA actually gets into the gel.
So, I need a better method which is quantitatively
reliable.Thank you in advance. My e-mail address is
benke2 at peds.miami.edu
Tammy J. Rossi
Research Clinical Specialist
Univ. Miami Sch. Med.