Single vs. Double Stranded DNA

Mark Walberg walberg at simmons.swmed.edu
Thu Nov 2 21:26:19 EST 1995


Ian Clarke <intermed at inforamp.net> wrote:
>Hi everyone:
>
>I am looking for a way to distinguish double stranded PCR products from 
>single stranded primers and the unincorporated dNTPs.
>
>Is it possible to do this by measuring the 260/280 ratio?
>
>Are there any other ways to distinguish these species without resorting 
>to column technologies.
>
>Thanks
------snip-----------------

I haven't tried it, but I sure bet that acridine orange staining would
 work very well.  Fluorescence is green for one and orange for the
other.  There is a paper in PNAS about how to do it for RNA vs DNA on 
glyoxal gels for Northern blots from anout 1977.
walberg at simmons.swmed.edu





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