Ian Clarke <intermed at inforamp.net> wrote:
>>I am looking for a way to distinguish double stranded PCR products from
>single stranded primers and the unincorporated dNTPs.
>>Is it possible to do this by measuring the 260/280 ratio?
>>Are there any other ways to distinguish these species without resorting
>to column technologies.
I haven't tried it, but I sure bet that acridine orange staining would
work very well. Fluorescence is green for one and orange for the
other. There is a paper in PNAS about how to do it for RNA vs DNA on
glyoxal gels for Northern blots from anout 1977.
walberg at simmons.swmed.edu