IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

Single vs. Double Stranded DNA

Mark Walberg walberg at simmons.swmed.edu
Thu Nov 2 21:26:19 EST 1995

Ian Clarke <intermed at inforamp.net> wrote:
>Hi everyone:
>I am looking for a way to distinguish double stranded PCR products from 
>single stranded primers and the unincorporated dNTPs.
>Is it possible to do this by measuring the 260/280 ratio?
>Are there any other ways to distinguish these species without resorting 
>to column technologies.

I haven't tried it, but I sure bet that acridine orange staining would
 work very well.  Fluorescence is green for one and orange for the
other.  There is a paper in PNAS about how to do it for RNA vs DNA on 
glyoxal gels for Northern blots from anout 1977.
walberg at simmons.swmed.edu

More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net