Re-using Wizard Minipreps

Morrison Lab fergusb at microbio.lifesci.ucla.edu
Thu Nov 2 20:57:50 EST 1995



I agree - syringes are re-usable if they are washed thoroughly in hot
saoapy water and bleach and rinsed thoroughly in distilled water but I
would not re use the little cartridges at all

Fergus

UCLA

In article <e-anderson-0211951126120001 at e-mac-56.ski.mskcc.org>,
e-anderson at ski.mskcc.org (Eric C. Anderson) wrote:

> In article <DH2DH0.DA2 at uns.bris.ac.uk>, bijgh at zeus.bris.ac.uk (JG. Head)
wrote:
> 
> jared,
> 
> in my old lab, we used to just stick the syringes in a 1-2L beaker of dH2O
> and when it was full we would wash them 1x in Alconox (or other lab
> detergent) and then 3 washes in dH2O followed by autoclaving.  no
> cross-contamination problems ever showed up.  (which is not to say that
> it's not possible, they just never happened.)
> 
> don't know about re-using the column filters but i don't think i would try.
> 
> eric
> 
> -- 
> Eric C. Anderson
> Cell Biology and Genetics
> Memorial Sloan-Kettering Cancer Center
> Sloan-Kettering Institute
> 1275 York Ave., Box 470
> New York, NY  10028
> Phone: (212)639-2977
> Fax:   (212)717-3298
> e-anderson at ski.mskcc.org



More information about the Methods mailing list