Purifying YAC DNA from PFGE gels
grierson at amc.uva.nl
Fri Nov 3 12:29:57 EST 1995
We are hoping to extract high enough quality YAC DNA (pure) from PFGE gels for
subsequent restriction digestion. Contemporary proeedure (apparently) is to
make a total library of yeast and YAC DNA, screen with COT-1, and pick these
clones. This seems a considerable amount of work.
So are there any decent protocols that get around this. How about Agarase (or
is that only for LMP agarose).
Any helpfull comments appreciated
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