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Purifying YAC DNA from PFGE gels

Andy Grierson grierson at amc.uva.nl
Fri Nov 3 12:29:57 EST 1995


We are hoping to extract high enough quality YAC DNA (pure) from PFGE gels for
subsequent restriction digestion.  Contemporary proeedure (apparently) is to
make a total library of yeast and YAC DNA, screen with COT-1, and pick these
clones.  This seems a considerable amount of work.  

So are there any decent protocols that get around this.  How about Agarase (or
is that only for LMP agarose).

Any helpfull comments appreciated


Andy Grierson
Neurology G2-108

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