After working with RAPDs and mites (which have so little DNA I cannot quantify)
I have finally found a few interesting items.
1. I have now three protocols that work for bees, blood and mites, if
anyone is interested, contact me.
2. I have found that spurious banding patterns will go away if the
annealing temperatures are raised from 37 to 40 or higher.
3. I am still having trouble extracting DNA from these mites. Can anyone
give me suggestions? I have tried the Phenol/chloroform, salt, and KoAC for
Drosophila. All work except the former. Bee DNA cleans up nicely with
dialysis but I loose too much mite DNA.
Thanks in adavance
Diana Sammataro, Ph.D. (now)
Ohio State Univeristy
1735 Neil Ave
Columbus, OH 43210 1220
dsammata at magnus.acs.ohio-state.edu