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Yeast PCR Advice

Ted Michelini tedm at darkwing.uoregon.edu
Sat Nov 4 00:52:40 EST 1995

In article <1995Nov2.091333.1 at mcvax2.d48.lilly.com>,
books at mcvax2.d48.lilly.com wrote:

> We are using a yeast two-hybrid system and would like to PCR the
positive yeast
> clones. I am told the yeast cell wall is diffcult to break open. Is there a
> yeast PCR prcedure or does anyone have advise for doing PCR with yeast? Thanks
> DPSMITH internet:smith_dennis_p at lilly.com

I've seen good results with PCR from whole yeast cells for recovery of
YAC's, the initial ~95°C denaturation seems to lyse enough of them to
work. I'm sure there is a ref., try medline. good luck.


Ted Michelini
Institute of Molecular Biology
University of Oregon
tedm at darkwing.uoregon.edu

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