Holger Bohnemeier (Bohnemeier at ukbf.fu-berlin.de) wrote:
: So I supose a) the sequences published for GAPDH mRNA (cDNA) and
: the corresponding genomic DNA are not correct
: (there might be polymorphisms laking 4 introns?),
: b) my Taq-Polymerase (GIBCO) has a slight RT-activity
: producing few cDNA molecules during the first steps of
: My question:
: Has anyone encountered with similar problems? Are there any ideas
: about this phenomenon (for me)?
Taq has been reported to have some RT-activity. I don't have the
references handy but could send them to you by e-mail if you are interested.
This subject has also been treated in this group previously, so you might
find some more information in the archive (e.g. gopher://net.bio.net).
Klaus Salger phone : +49 (0)89 5902 -502
Zoologisches Institut FAX : -450
AG MacWilliams e-mail: salger at zi.biologie.uni-muenchen.de