Protein blotted membrane storage before probing or .. but how?

Markus Piotrowski Markus.Piotrowski at rz.ruhr-uni-bochum.de
Fri Nov 3 16:03:27 EST 1995


jencsa at net.sote.hu (Jeney Csaba) wrote:
>Hi,
>
>I like to perform a series of experiments which result in a lot of SDS-PAGE
>gels. First I shall use a radioactive method to evaluate them, but for
>confirmation purposes I need to probe them with Mabs according to the Western
>technique later. So the question is how can I store the samples (in gel form or
>after blotted them to a membrane) without deterioration?
>
>Thanks in advance
>
>Csaba


Dear Jeney,

after blotting and maybe staining with Ponceau-S we dry our membranes
between blotting paper and store them at -20°C.

Best wishes, Markus




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