Single vs. Double Stranded DNA
Guy Plunkett III
guy at genetics.wisc.edu
Fri Nov 3 17:26:18 EST 1995
In article <47bukb$ako at swsu65.swmed.edu>, Mark Walberg
<walberg at simmons.swmed.edu> wrote:
> I haven't tried it, but I sure bet that acridine orange staining would
> work very well. Fluorescence is green for one and orange for the
> other. There is a paper in PNAS about how to do it for RNA vs DNA on
> glyoxal gels for Northern blots from anout 1977.
Here are references for acridine orange staining:
 G. K. McMaster and G. C. Carmichael (1977) Analysis of single- and
double-stranded nucleic acids on polyacrylamide and agarose gels by using
glyoxal and acridine orange. Proc. Natl. Acad. Sci. U.S.A. 74, 48354838.
 G. C. Carmichael and G. K. McMaster (1980) The analysis of nucleic acids
in gels using glyoxal and acridine orange. Methods Enzymol. 65, 380391.
 R. C. Ogden and D. A. Adams (1987) Electrophoresis in agarose and
acrylamide gels. Methods Enzymol. 152, 6187.
Dr.Guy Plunkett III for the E. coli Genome Project
Laboratory of Genetics
University of Wisconsin Phone: (608) 262-2534
445 Henry Mall Fax: (608) 263-7459
Madison, WI 53706 Internet: guy at genetics.wisc.edu
"Life is too short, and DNA too long."
-- Michael Crichton, Jurassic Park
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