Single vs. Double Stranded DNA

Guy Plunkett III guy at
Fri Nov 3 17:26:18 EST 1995

In article <47bukb$ako at>, Mark Walberg
<walberg at> wrote:

> I haven't tried it, but I sure bet that acridine orange staining would
>  work very well.  Fluorescence is green for one and orange for the
> other.  There is a paper in PNAS about how to do it for RNA vs DNA on 
> glyoxal gels for Northern blots from anout 1977.

Here are references for acridine orange staining:

[1] G. K. McMaster and G. C. Carmichael (1977) Analysis of single- and
double-stranded nucleic acids on polyacrylamide and agarose gels by using
glyoxal and acridine orange. Proc. Natl. Acad. Sci. U.S.A. 74, 4835­4838.

[2] G. C. Carmichael and G. K. McMaster (1980) The analysis of nucleic acids
in gels using glyoxal and acridine orange. Methods Enzymol. 65, 380­391.

[3] R. C. Ogden and D. A. Adams (1987) Electrophoresis in agarose and
acrylamide gels. Methods Enzymol. 152, 61­87.

Cheers, GUY

Dr.Guy Plunkett III for the E. coli Genome Project
Laboratory of Genetics
University of Wisconsin        Phone: (608) 262-2534
445 Henry Mall                 Fax:   (608) 263-7459
Madison, WI  53706             Internet: guy at

                "Life is too short, and DNA too long."
                  -- Michael Crichton, Jurassic Park

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