Does anyone have any ideas on how to check if you have DNA on a colony lift
membrane without staining the membrane with ethidium bromide or some other
such nasty? We have recently changed membranes and are not getting the same
numbers of probe positives in some of our samples as previously. We also need
to probe these same membranes later, so nothing destructive. Any comments or
suggestions would be greatly appreciated.
Biotechnology Research Institute
National Research Council of Canada