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DNA extraction of 140 kb piece from agarose

Christina Chan bm335 at FreeNet.Carleton.CA
Sat Nov 4 15:47:51 EST 1995

I have been having difficulty in isolating a 140 kb piece of DNA
from a TBE agarose (1% SeaKem GTG) plug.  The plug contains
~500 ng of DNA.  I have tried the Gene Clean II kit but was un-

Any suggestions?

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