Q : Sequencer
aaron jacob kelly
ajkelly at silver.ucs.indiana.edu
Sun Nov 5 12:27:10 EST 1995
our group is verry interested in an automatic sequencer. So we would like to
get some information from peoples who have some expirience with the
"Alf-Express"-Sequencer from Pharmacia and the Li-Cor-Sequencer.
Thanks a lot for your help !
we have access to a li-cor sequencer in our department and i have used it
alot with great success using labeled primers. i personally have not had
much success with internal labeling, but i was using a new protocol.
someone else in our lab has gotten that to work as well.
the larger model seemed to give much better results than the shorter
one. i have typically gotten around 800 basepairs of pretty good read
using just the autosequencing with no manual editing. i have gotten up
to 1000 basepairs with only a few ambiguities, but 650 of that was vector.
we use the sequitherm kit from epicenter technologies.
ajkelly at ucs.indiana.edu
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