In article <475mej$r6u at sifon.cc.mcgill.ca>, Petros, BKXO at musicb.mcgill.ca wrote:
> I would like to differentiate by gel electrophoresis between DNA
> fragments differing in size by as little as a few base pairs. The size of
> the DNA fragments are between 500-900bp and some differ by as little
> as 5bp.
>> Can anyone suggest a high resolution mini-gel system that might
> accomplish separation of DNA fragments differing in size by as little
> as 5bp?
Try and contact FMC, they have a few formulations of agarose that will do
high resolution. (sorry don't have their contact info handy)
I think one of their formulations (Metaphor?) claims a 2 bp resolution
The alternative would be to use acrylamide
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