High Resolution mini-gels?

gustilo at pobox.upenn.edu gustilo at pobox.upenn.edu
Sun Nov 5 16:58:15 EST 1995


In article <475mej$r6u at sifon.cc.mcgill.ca>, Petros, BKXO at musicb.mcgill.ca wrote:

> I would like to differentiate by gel electrophoresis between DNA 
> fragments differing in size by as little as a few base pairs.  The size of 
> the DNA fragments are between 500-900bp and some differ by as little 
> as 5bp.
> 
> Can anyone suggest a high resolution mini-gel system that might
> accomplish separation of DNA fragments differing in size by as little 
> as 5bp?

Try and contact FMC, they have a few formulations of agarose that will do
high resolution. (sorry don't have their contact info handy)

I think one of their formulations (Metaphor?) claims a 2 bp resolution

The alternative would be to use acrylamide

--

Bicycle Crash Test Dummy for Hire
gustilo at pobox.upenn.edu



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